LOCUS       M50 Super 8x TOPFlash	4997 bp 	DNA	SYN
DEFINITION  M50 Super 8x TOPFlash
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="M50 Super 8x TOPFlash"
                     /mol_type="other DNA"
     gene            267..1919
                     /label="firefly_Luciferase"
                     /gene="firefly_Luciferase"
     CDS             267..1919
                     /label="ORF frame 3"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     misc_feature    complement(333..351)
                     /label="LucNrev_primer"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     misc_feature    complement(1971..1990)
                     /label="EBV_rev_primer"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     gene            complement(3259..4119)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     CDS             complement(3259..4119)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(4161..4189)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4268..4574
                     /label="f1_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             complement(4784..404)
                     /label="ORF frame 1"
                     /translation="MCICKSNCSRNQGVSLHSLMQLLSSGSIFQRIEWRRAFLYVFGV
                     FHGGFTNSTGMPSFLPTVPECQAGIRASIIYPLESRSRARASTRARPFDLTPFDLTPF
                     DLTPFDLAPLILPPLILPPLILPPLISRKSSVPIDREMFWHLHLHWGQPILLVCFVSF
                     CFDGERMLVLSIHTKNQHTDVMKIKIFYCGRSKYLPYLNITYLSW*"
     misc_feature    4837..4990
                     /label="TB"
                     /translation="MCICKSNCSRNQGVSLHSLMQLLSSGSIFQRIEWRRAFLYVFGV
                     FHGGFTNSTGMPSFLPTVPECQAGIRASIIYPLESRSRARASTRARPFDLTPFDLTPF
                     DLTPFDLAPLILPPLILPPLILPPLISRKSSVPIDREMFWHLHLHWGQPILLVCFVSF
                     CFDGERMLVLSIHTKNQHTDVMKIKIFYCGRSKYLPYLNITYLSW*"
     terminator      4837..4885
                     /label="synth_PA_terminator"
                     /translation="MCICKSNCSRNQGVSLHSLMQLLSSGSIFQRIEWRRAFLYVFGV
                     FHGGFTNSTGMPSFLPTVPECQAGIRASIIYPLESRSRARASTRARPFDLTPFDLTPF
                     DLTPFDLAPLILPPLILPPLILPPLISRKSSVPIDREMFWHLHLHWGQPILLVCFVSF
                     CFDGERMLVLSIHTKNQHTDVMKIKIFYCGRSKYLPYLNITYLSW*"
     terminator      4837..4990
                     /label="up_PA_terminator"
                     /translation="MCICKSNCSRNQGVSLHSLMQLLSSGSIFQRIEWRRAFLYVFGV
                     FHGGFTNSTGMPSFLPTVPECQAGIRASIIYPLESRSRARASTRARPFDLTPFDLTPF
                     DLTPFDLAPLILPPLILPPLILPPLISRKSSVPIDREMFWHLHLHWGQPILLVCFVSF
                     CFDGERMLVLSIHTKNQHTDVMKIKIFYCGRSKYLPYLNITYLSW*"
     misc_feature    4939..4958
                     /label="RVprimer3_primer"
                     /translation="MCICKSNCSRNQGVSLHSLMQLLSSGSIFQRIEWRRAFLYVFGV
                     FHGGFTNSTGMPSFLPTVPECQAGIRASIIYPLESRSRARASTRARPFDLTPFDLTPF
                     DLTPFDLAPLILPPLILPPLILPPLISRKSSVPIDREMFWHLHLHWGQPILLVCFVSF
                     CFDGERMLVLSIHTKNQHTDVMKIKIFYCGRSKYLPYLNITYLSW*"
ORIGIN
    1 ggtaccgagc tcttacgcga gatcaaaggg ggtaagatca aagggggtaa gatcaaaggg
   61 ggtaagatca aaggcgcgag atcaaagggg gtaagatcaa agggggtaag atcaaagggg
  121 gtaagatcaa aggggcgcgc ccgcgtgcta gcccgggctc gagatctaga ctctagaggg
  181 tatataatgg aagctcgaat tccagcttgg cattccggta ctgttggtaa aaagcttggc
  241 attccggtac tgttggtaaa gccaccatgg aagacgccaa aaacataaag aaaggcccgg
  301 cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga
  361 gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca
  421 cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc
  481 tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg
  541 tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac
  601 gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg
  661 ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca
  721 tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc
  781 tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa
  841 ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc
  901 atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca
  961 ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta
 1021 cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc
 1081 tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat
 1141 tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa
 1201 ttgcttctgg tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc
 1261 atctgccagg tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta
 1321 cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga
 1381 aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg
 1441 tgagaggtcc tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga
 1501 ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact
 1561 tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg
 1621 ctgaattgga atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc
 1681 ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga
 1741 cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt
 1801 tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg
 1861 caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgtaat
 1921 tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
 1981 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
 2041 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
 2101 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
 2161 acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc ccttgagagc cttcaaccca
 2221 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
 2281 tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga
 2341 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
 2401 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
 2461 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
 2521 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
 2581 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
 2641 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
 2701 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
 2761 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
 2821 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
 2881 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
 2941 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
 3001 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
 3061 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
 3121 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
 3181 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
 3241 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
 3301 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
 3361 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
 3421 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
 3481 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
 3541 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
 3601 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
 3661 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
 3721 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
 3781 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
 3841 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
 3901 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
 3961 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
 4021 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
 4081 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
 4141 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
 4201 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg
 4261 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
 4321 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
 4381 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
 4441 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
 4501 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
 4561 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
 4621 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
 4681 taacaaaata ttaacgttta caatttccca ttcgccattc aggctgcgca actgttggga
 4741 agggcgatcg gtgcgggcct cttcgctatt acgccagccc aagctaccat gataagtaag
 4801 taatattaag gtacgggagg tacttggagc ggccgcaata aaatatcttt attttcatta
 4861 catctgtgtg ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac
 4921 aaaacgaaac aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga
 4981 acatttctct atcgata
//