LOCUS       pFLAG-CMV-2	4679 bp 	DNA   circular	SYN
DEFINITION  pFLAG-CMV-2
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="pFLAG-CMV-2"
                     /mol_type="other DNA"
     misc_feature    complement(12..155)
                     /label="lacZ_a"
     misc_feature    126..148
                     /label="M13_pUC_fwd_primer"
     promoter        141..157
                     /label="M13_forward20_primer"
     promoter        319..895
                     /label="CMV_immearly_promoter"
     misc_feature    398..685
                     /label="CAG_enhancer"
     promoter        841..912
                     /label="CMV_promoter"
     misc_feature    852..872
                     /label="CMV_fwd_primer"
     misc_feature    896..915
                     /label="pCEP_fwd_primer"
     terminator      1030..1496
                     /label="hGH_PA_terminator"
     misc_feature    complement(1666..1686)
                     /label="pBABE_3_primer"
     misc_feature    complement(1672..1887)
                     /label="SV40_enhancer"
     promoter        1684..1952
                     /label="SV40_promoter"
     rep_origin      1851..1928
                     /label="SV40_origin"
     misc_feature    1913..1932
                     /label="SV40pro_F_primer"
     promoter        complement(2039..2057)
                     /label="T7_promoter"
     misc_feature    complement(2084..2106)
                     /label="M13_pUC_rev_primer"
     promoter        complement(2120..2149)
                     /label="lac_promoter"
     gene            complement(3232..4092)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(3232..4092)
                     /label="ORF frame 3"
     promoter        complement(4134..4162)
                     /label="AmpR_promoter"
     rep_origin      4241..4547
                     /label="f1_origin"
ORIGIN
    1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
   61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
  121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
  181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
  241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
  301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
  361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
  421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
  481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
  541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
  601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
  661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
  721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
  781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
  841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
  901 tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg acgacaagct
  961 tgcggccgcg aattcatcga tagatctgat atcggtacca gtcgactcta gaggatcccg
 1021 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
 1081 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
 1141 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggaagacaa
 1201 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
 1261 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
 1321 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
 1381 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
 1441 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctga
 1501 ttttaaaata actataccag caggaggacg tccagacaca gcataggcta cctggccatg
 1561 cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc gttgggtcca
 1621 ctcagtagat gcctgttgaa ttgggtacgc ggccagcttg gctgtggaat gtgtgtcagt
 1681 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
 1741 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
 1801 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
 1861 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
 1921 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
 1981 gaggcctagg cttttgcaaa aagctcctcg aggaactgaa aaaccagaaa gttaattccc
 2041 tatagtgagt cgtattaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta
 2101 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
 2161 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
 2221 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
 2281 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
 2341 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
 2401 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
 2461 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
 2521 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
 2581 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
 2641 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
 2701 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
 2761 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
 2821 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
 2881 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
 2941 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
 3001 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
 3061 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
 3121 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
 3181 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
 3241 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
 3301 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
 3361 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
 3421 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
 3481 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
 3541 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
 3601 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
 3661 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
 3721 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
 3781 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
 3841 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
 3901 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
 3961 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
 4021 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
 4081 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
 4141 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
 4201 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
 4261 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
 4321 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
 4381 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
 4441 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
 4501 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
 4561 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
 4621 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattt

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