pDual-GC

创建日期:2014-10-01 查看:118
  • 出品公司 Agilent
  • 其他名称
  • 载体类型 真核原核双系统表达载体;克隆载体
  • 表达水平
  • 克隆方法 多克隆位点,限制性内切酶
  • 载体大小 6640 bp
  • 5'端测序 --
  • 3'端测序 --
  • 载体标签 His tag ;CPB tag
  • 载体抗性 氨苄青霉素
  • 稳定性 --
  • 组成
  • 病毒/非病毒 非病毒
pDual GC, 6640 bp                              version 027003

NOTE: The following sequence has been verified for accuracy
at the junctions. The remainder of the sequence has been 
obtained from existing data. 


    1  GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA
   51  ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT
  101  TCAATAATAT TGAAAAAGGA AGAATCCTGA GGCGGAAAGA ACCAGCTGTG
  151  GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA
  201  GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG
  251  TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA
  301  GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC
  351  CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT
  401  TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG
  451  AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAGATCGA TCAAGAGACA
  501  GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC
  551  TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA
  601  CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC
  651  CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA
  701  AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  751  CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG
  801  GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA
  851  GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC
  901  CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA
  951  CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA
 1001  ACATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
 1051  TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG
 1101  AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG
 1151  GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA
 1201  TTGCTGAAGA ACTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC
 1251  GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA
 1301  CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GAAATGACCG ACCAAGCGAC
 1351  GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA
 1401  GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG
 1451  CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCTAGGG GGAGGCTAAC
 1501  TGAAACACGG AAGGAGACAA TACCGGAAGG AACCCGCGCT ATGACGGCAA
 1551  TAAAAAGACA GAATAAAACG CACGGTGTTG GGTCGTTTGT TCATAAACGC
 1601  GGGGTTCGGT CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA
 1651  TTGGGGCCAA TACGCCCGCG TTTCTTCCTT TTCCCCACCC CACCCCCCAA
 1701  GTTCGGGTGA AGGCCCAGGG CTCGCAGCCA ACGTCGGGGC GGCAGGCCCT
 1751  GCCATAGCCT CAGGTTACTC ATATATACTT TAGATTGATT TAAAACTTCA
 1801  TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA
 1851  CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA
 1901  GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG
 1951  CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG
 2001  ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG
 2051  CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT
 2101  CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC
 2151  CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA
 2201  AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC
 2251  GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC
 2301  TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG
 2351  GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA
 2401  GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC
 2451  ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC
 2501  CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG
 2551  CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG
 2601  ATAACCGTAT TACCGTAATG AGTGAGCTAA CTTACATTAA TTGCGTTGCG
 2651  CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT
 2701  GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCCAGGGT
 2751  GGTTTTTCTT TTCACCAGTG AGACGGGCAA CAGCTGATTG CCCTTCACCG
 2801  CCTGGCCCTG AGAGAGTTGC AGCAAGCGGT CCACGCTGGT TTGCCCCAGC
 2851  AGGCGAAAAT CCTGTTTGAT GGTGGTTAAC GGCGGGATAT AACATGAGCT
 2901  GTCTTCGGTA TCGTCGTATC CCACTACCGA GATATCCGCA CCAACGCGCA
 2951  GCCCGGACTC GGTAATGGCG CGCATTGCGC CCAGCGCCAT CTGATCGTTG
 3001  GCAACCAGCA TCGCAGTGGG AACGATGCCC TCATTCAGCA TTTGCATGGT
 3051  TTGTTGAAAA CCGGACATGG CACTCCAGTC GCCTTCCCGT TCCGCTATCG
 3101  GCTGAATTTG ATTGCGAGTG AGATATTTAT GCCAGCCAGC CAGACGCAGA
 3151  CGCGCCGAGA CAGAACTTAA TGGGCCCGCT AACAGCGCGA TTTGCTGGTG
 3201  ACCCAATGCG ACCAGATGCT CCACGCCCAG TCGCGTACCG TCTTCATGGG
 3251  AGAAAATAAT ACTGTTGATG GGTGTCTGGT CAGAGACATC AAGAAATAAC
 3301  GCCGGAACAT TAGTGCAGGC AGCTTCCACA GCAATGGCAT CCTGGTCATC
 3351  CAGCGGATAG TTAATGATCA GCCCACTGAC GCGTTGCGCG AGAAGATTGT
 3401  GCACCGCCGC TTTACAGGCT TCGACGCCGC TTCGTTCTAC CATCGACACC
 3451  ACCACGCTGG CACCCAGTTG ATCGGCGCGA GATTTAATCG CCGCGACAAT
 3501  TTGCGACGGC GCGTGCAGGG CCAGACTGGA GGTGGCAACG CCAATCAGCA
 3551  ACGACTGTTT GCCCGCCAGT TGTTGTGCCA CGCGGTTGGG AATGTAATTC
 3601  AGCTCCGCCA TCGCCGCTTC CACTTTTTCC CGCGTTTTCG CAGAAACGTG
 3651  GCTGGCCTGG TTCACCACGC GGGAAACGGT CTGATAAGAG ACACCGGCAT
 3701  ACTCTGCGAC ATCGTATAAC GTTACTGGTT TCACATTCAC CACCCTGAAT
 3751  TGACTCTCTT TCGGGCGCTA TCATGCCATA CCGCGAAAGG TTTTGCGCCA
 3801  TTCGATTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA
 3851  TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC
 3901  CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA
 3951  GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG
 4001  GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC
 4051  CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG
 4101  TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT
 4151  CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG
 4201  GATAGCGGTT TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC
 4251  AATGGGAGTT TGTTTTGGCA CCAAAATCAA CGGGACTTTC CAAAATGTCG
 4301  TAACAACTCC GCCCCATTGA CGCAAATGGG CGGTAGGCGT GCCTAATGGG
 4351  AGGTCTATAT AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCTA
 4401  ATACGACTCA CTATAGGGGA ATTGTGAGCG GATAACAATT CCCCTTGTTT
 4451  AAACTTTAAG AGGAGGGCCA CCATGGGAAG AGAATTCGTG CGCGGAACCC
 4501  CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA
 4551  CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA GGAGTATGAG
 4601  TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC
 4651  TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA
 4701  GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG
 4751  TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA
 4801  CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG
 4851  CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA
 4901  GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG
 4951  AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA
 5001  CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA
 5051  CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG
 5101  AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA
 5151  ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG
 5201  GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC
 5251  TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC
 5301  GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA
 5351  GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG
 5401  ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT
 5451  TGGTAACTGT CAGACCAAGT TTACTCATAT ATAGAATTCA AGCTTTCCTC
 5501  TTCACTTAGT TTAAACACTG CGGCCGCGCT GCTGGTTCCG CGTGGTTCTA
 5551  CTAGTGAGCA GAAACTCATC TCTGAAGAAG ATCTGGAACA AAAGTTGATT
 5601  TCAGAAGAAG ATCTGGAACA GAAGCTCATC TCTGAGGAAG ATCTGGGTAC
 5651  CGCTGGCTCC GCTGCTGGTT CTAGACATCA CCATCACCAT CACTAATGAT
 5701  AATCCGCGTG GTTTCCAATA CCCAGCTTTG ACTTGACTTG ATAACAGGTA
 5751  AGTGTACCCG GATCCGGGGA ATTGTGAGCG GATAACTTCC CCGATCCGCC
 5801  CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGAGATC CAATTTTTAA
 5851  GTGTATAATG TGCTAAACTA CTGATTCTAA TTGTTTGTGT ATTTTAGATT
 5901  CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT
 5951  GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT
 6001  TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT
 6051  CATTTTATGT TTCAGGTTCA GGGGGAGATG TGGGAGGTTT TTTAAAGCAA
 6101  GTAAAACCTC TACAAATGTG GTAACGCGTA TAACCCCTTG GGGCCTCTAA
 6151  ACGGGTCTTG AGGGGTTTTT TGACGCGTAA ATTGTAAGCG TTAATATTTT
 6201  GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT
 6251  AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA
 6301  GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT
 6351  GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC
 6401  TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA
 6451  GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG
 6501  AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG
 6551  GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA
 6601  CCCGCCGCGC TTAATGCGCC GCTACAGGGC GCGTCAGGTG


科研狗©2015-2024 科研好助手,京ICP备20005780号-1 建议意见

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