LOCUS       pBI-CMV4	3779 bp 	DNA	SYN
DEFINITION  pBI-CMV4
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="pBI-CMV4"
                     /mol_type="other DNA"
     promoter        4..568
                     /label="CMV_immearly_promoter"
     misc_feature    71..358
                     /label="CAG_enhancer"
     misc_feature    525..545
                     /label="CMV_fwd_primer"
     promoter        526..595
                     /label="CMV_promoter"
     gene            complement(1799..2659)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(1799..2659)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(2701..2729)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             complement(2987..3778)
                     /label="ORF frame 2"
                     /translation="MSRWRVRWEAYISRARLVNRQIAWRIRHHGLLRERHHRVHALQG
                     AHGGHRERPRVRDRGRGRGPPLRGPQHREAEGDQGRPPALRLGHPVPPVPVRLQGVRE
                     APRRHPRLQEAVLPRGLQVGARDELRGRRRGDRDPGLLPAGRLLHLQGEVHRRELPLR
                     RPRDAEEDHGLGGLHRAPVPPRRRAEGRDPQGPEAEGRRPLPGGVQVHLHGQEARAAA
                     RLLLRGRQAGHHLPQRGLHHRGAVRAHRGPPPPVPVGPRSPEAAV*"
     CDS             complement(3016..3693)
                     /label="ORF frame 3"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDG
                     GVATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGE
                     THKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTE
                     GRHHLFL*"
     misc_feature    complement(3078..3101)
                     /label="dsRed1_C_primer"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDG
                     GVATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGE
                     THKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTE
                     GRHHLFL*"
     misc_feature    3493..3513
                     /label="dsRed1_N_primer"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDG
                     GVATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGE
                     THKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTE
                     GRHHLFL*"
     misc_feature    complement(3713..3737)
                     /label="LNCX_primer"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDG
                     GVATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGE
                     THKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTE
                     GRHHLFL*"
     misc_feature    complement(3720..3739)
                     /label="pCEP_fwd_primer"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDG
                     GVATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGE
                     THKALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTE
                     GRHHLFL*"
ORIGIN
    1 ctcgagtagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
   61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
  121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
  181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
  241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
  301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
  361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
  421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
  481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
  541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
  601 gggatcctct agtcagctga cgcgtgctag cgcggccgca tcgataagct tgtcgacgat
  661 atctccagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
  721 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
  781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
  841 aagcattttt ttcactgccc cgagcttcct cgctcactga ctcgctgcgc tcggtcgttc
  901 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
  961 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
 1021 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
 1081 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
 1141 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
 1201 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
 1261 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
 1321 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
 1381 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
 1441 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
 1501 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
 1561 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
 1621 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
 1681 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
 1741 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
 1801 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
 1861 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
 1921 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
 1981 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
 2041 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
 2101 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
 2161 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
 2221 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
 2281 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
 2341 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
 2401 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
 2461 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
 2521 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
 2581 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
 2641 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
 2701 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
 2761 cgcgcacatt tccccgaaaa gtgccacctg acgtcggcag tgaaaaaaat gctttatttg
 2821 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
 2881 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
 2941 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag actgcagcct
 3001 caggagatct gggccctaca ggaacaggtg gtggcggccc tcggtgcgct cgtactgctc
 3061 cacgatggtg tagtcctcgt tgtgggaggt gatgtccagc ttggcgtcca cgtagtagta
 3121 gccgggcagc tgcacgggct tcttggccat gtagatggac ttgaactcca ccaggtagtg
 3181 gccgccgtcc ttcagcttca gggccttgtg ggtctcgccc ttcagcacgc cgtcgcgggg
 3241 gtacaggcgc tcggtggagg cctcccagcc catggtcttc ttctgcatca cggggccgtc
 3301 ggaggggaag ttcacgccga tgaacttcac cttgtagatg aagcagccgt cctgcaggga
 3361 ggagtcctgg gtcacggtcg ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt
 3421 gaagccctcg gggaaggaca gcttcttgta gtcggggatg tcggcggggt gcttcacgta
 3481 caccttggag ccgtactgga actgggggga caggatgtcc caggcgaagg gcagggggcc
 3541 gcccttggtc accttcagct tcacggtgtt gtggccctcg taggggcggc cctcgccctc
 3601 gccctcgatc tcgaactcgt ggccgttcac ggtgccctcc atgcgcacct tgaagcgcat
 3661 gaactcggtg atgacgttct cggaggaggc catggtggcg aattctccag gcgatctgac
 3721 ggttcactaa acgagctctg cttatatagg cctcccaccg tacacgccac ctcgacata

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