LOCUS       pBridge    6526 bp    DNA    SYN    30-Oct-2013
DEFINITION  pBridge
ACCESSION    
KEYWORDS    
SOURCE    
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="pBridge"
                     /mol_type="other DNA"
     promoter        10..406
                     /label="yADH1_promoter"
     misc_feature    434..874
                     /label="Gal4_DBD"
     misc_feature    838..856
                     /label="Gal4_N_term_primer"
     misc_feature    complement(976..1000)
                     /label="pHybLex_rev_primer"
     terminator      921..1112
                     /label="yADH1_terminator"
     CDS             complement(1161..1835)
                     /label="TRP1"
                     /gene="TRP1"
                     /note="ORF frame 3"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNR
                     KRTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGD
                     ESWQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSI
                     SDWVGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANF
                     VKNAKK*"
     gene            complement(1161..1835)
                     /label="TRP1"
                     /gene="TRP1"
     misc_feature    complement(1964..1986)
                     /label="M13_pUC_rev_primer"
     promoter        complement(2000..2029)
                     /label="lac_promoter"
     rep_origin      complement(3341..3960)
                     /label="pBR322_origin"
     CDS             complement(4115..4975)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /note="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     gene            complement(4115..4975)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     promoter        complement(5017..5045)
                     /label="AmpR_promoter"
     misc_feature    complement(5204..5226)
                     /label="pGEX_3_primer"
     rep_origin      5362..6526
                     /label="2micron2_origin"
ORIGIN
    1 GCTTGCATGC AACTTCTTTT CTTTTTTTTT CTTTTCTCTC TCCCCCGTTG TTGTCTCACC 
   61 ATATCCGCAA TGACAAAAAA ATGATGGAAG ACACTAAAGG AAAAAATTAA CGACAAAGAC 
  121 AGCACCAACA GATGTCGTTG TTCCAGAGCT GATGAGGGGT ATCTCGAAGC ACACGAAACT 
  181 TTTTCCTTCC TTCATTCACG CACACTACTC TCTAATGAGC AACGGTATAC GGCCTTCCTT 
  241 CCAGTTACTT GAATTTGAAA TAAAAAAAAG TTTGCTGTCT TGCTATCAAG TATAAATAGA 
  301 CCTGCAATTA TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT 
  361 CTTTTTCTGC ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCCAA GCTTGAAGCA 
  421 AGCCTCCTGA AAGATGAAGC TACTGTCTTC TATCGAACAA GCATGCGATA TTTGCCGACT 
  481 TAAAAAGCTC AAGTGCTCCA AAGAAAAACC GAAGTGCGCC AAGTGTCTGA AGAACAACTG 
  541 GGAGTGTCGC TACTCTCCCA AAACCAAAAG GTCTCCGCTG ACTAGGGCAC ATCTGACAGA 
  601 AGTGGAATCA AGGCTAGAAA GACTGGAACA GCTATTTCTA CTGATTTTTC CTCGAGAAGA 
  661 CCTTGACATG ATTTTGAAAA TGGATTCTTT ACAGGATATA AAAGCATTGT TAACAGGATT 
  721 ATTTGTACAA GATAATGTGA ATAAAGATGC CGTCACAGAT AGATTGGCTT CAGTGGAGAC 
  781 TGATATGCCT CTAACATTGA GACAGCATAG AATAAGTGCG ACATCATCAT CGGAAGAGAG 
  841 TAGTAACAAA GGTCAAAGAC AGTTGACTGT ATCGCCGGAA TTCCCGGGGA TCCGTCGACC 
  901 TGCAGCCAAG CTAATTCCGG GCGAATTTCT TATGATTTAT GATTTTTATT ATTAAATAAG 
  961 TTATAAAAAA AATAAGTGTA TACAAATTTT AAAGTGACTC TTAGGTTTTA AAACGAAAAT 
 1021 TCTTGTTCTT GAGTAACTCT TTCCTGTAGG TCAGGTTGCT TTCTCAGGTA TAGCATGAGG 
 1081 TCGCTCTTAT TGACCACACC TCTACCGGCA TGCCGGCAAG TGCACAAACA ATACTTAAAT 
 1141 AAATACTACT CAGTAATAAC CTATTTCTTA GCATTTTTGA CGAAATTTGC TATTTTGTTA 
 1201 GAGTCTTTTA CACCATTTGT CTCCACACCT CCGCTTACAT CAACACCAAT AACGCCATTT 
 1261 AATCTAAGCG CATCACCAAC ATTTTCTGGC GTCAGTCCAC CAGCTAACAT AAAATGTAAG 
 1321 CTTTCGGGGC TCTCTTGCCT TCCAACCCAG TCAGAAATCG AGTTCCAATC CAAAAGTTCA 
 1381 CCTGTCCCAC CTGCTTCTGA ATCAAACAAG GGAATAAACG AATGAGGTTT CTGTGAAGCT 
 1441 GCACTGAGTA GTATGTTGCA GTCTTTTGGA AATACGAGTC TTTTAATAAC TGGCAAACCG 
 1501 AGGAACTCTT GGTATTCTTG CCACGACTCA TCTCCATGCA GTTGGACGAT ATCAATGCCG 
 1561 TAATCATTGA CCAGAGCCAA AACATCCTCC TTAGGTTGAT TACGAAACAC GCCAACCAAG 
 1621 TATTTCGGAG TGCCTGAACT ATTTTTATAT GCTTTTACAA GACTTGAAAT TTTCCTTGCA 
 1681 ATAACCGGGT CAATTGTTCT CTTTCTATTG GGCACACATA TAATACCCAG CAAGTCAGCA 
 1741 TCGGAATCTA GAGCACATTC TGCGGCCTCT GTGCTCTGCA AGCCGCAAAC TTTCACCAAT 
 1801 GGACCAGAAC TACCTGTGAA ATTAATAACA GACATACTCC AAGCTGCCTT TGTGTGCTTA 
 1861 ATCACGTATA CTCACGTGCT CAATAGTCAC CAATGCCCTC CCTCTTGGCC CTCTCCTTTT 
 1921 CTTTTTTCGA CCGAATTAAT TCGTAATCAT GTCATAGCTG TTTCCTGTGT GAAATTGTTA 
 1981 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC 
 2041 CTAATGAGTG AGGTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG 
 2101 AAACCTGTCG TGCCAGGAAG ATCCGAGGCC TAGCTTCTAA TTCTTCCAAC ATACAATGGG 
 2161 AGTTTGGCCG AGTGGTTTAA GGCGTCAGAT TTAGGTGGAT TTAACCTCTA AAATCTCTGA 
 2221 TATCTTCGGA TGCAAGGGTT CGAATCCCTT AGCTCTCATT ATTTTTTGCT TTTTCTCTTG 
 2281 AGGTCACATG ATCGCAAAAT GGCAAATGGC ACGTGAAGCT GTCGATATTG GGGAACTGTG 
 2341 GTGGTTGGCA AATGACTAAT TAAGTTAGTC AAGGCGCCAT CCTCATGAAA ACTGTGTAAC 
 2401 ATAATAACCG AAGTGTCGAA AAGGTGGCAC CTTGTCCAAT TGAACACGCT CGATGAAAAA 
 2461 AATAAGATAT ATATAAGGTT AAGTAAAGCG TCTGTTAGAA AGGAAGTTTT TCCTTTTTCT 
 2521 TGCTCTCTTG TCTTTTCATC TACTATTTCC TTCGTGTAAT ACAGGGTCGT CAGATACATA 
 2581 GATACAATTC TATTACCCCC ATCCATACAA TGGGCCATAT GGCTTCTAGC TATCCTTATG 
 2641 ACGTGCCTGA CTATGCCAGC CTGGGAGGAC CTTCTAGTCC TAAGAAGAAG AGAAAGGTGG 
 2701 CGGCCGCATT AGCCCGAAGA TCTTCGGGCT GATCTCCCAT GTCTCTACTG GTGGTGGTGC 
 2761 TTCTTTGGAA TTATTGGAAG GTAAGGAATT GCCAGGTGTT GCTTTCTTAT CCGAAAAGAA 
 2821 ATAAATTGAA TTGAATTGAA ATCGATAGAT CAATTTTTTT CTTTTCTCTT TCCCCATCCT 
 2881 TTACGCTAAA ATAATAGTTT ATTTTATTTT TTGAATATTT TTTATTTATA TACGTATATA 
 2941 TAGACTATTA TTTATCTTTT AATGATTATT AAGATTTTTA TTAAAAAAAA ATTCGCTCCT 
 3001 CTTTTAATGC CTTTATGCAG TTTTTTTTTC CCATTCGATA TTTCTATGTT CGGGTTCAGC 
 3061 GTATTTTAAG TTTAATAACT CGAAAATTCT GCGTTCGTTA AAGCTAGGCC TCGGATCTTC 
 3121 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGTTT GCGTATTGGG CGCTCTTCCG 
 3181 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 
 3241 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 
 3301 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 
 3361 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 
 3421 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 
 3481 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 
 3541 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 
 3601 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 
 3661 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 
 3721 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 
 3781 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 
 3841 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT 
 3901 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 
 3961 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 
 4021 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 
 4081 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 
 4141 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 
 4201 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 
 4261 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 
 4321 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 
 4381 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 
 4441 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 
 4501 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 
 4561 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 
 4621 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 
 4681 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 
 4741 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 
 4801 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 
 4861 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 
 4921 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 
 4981 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 
 5041 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 
 5101 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA 
 5161 CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC 
 5221 TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG 
 5281 GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA 
 5341 TTGTACTGAG AGTGCACCAT AACGCATTTA AGCATAAACA CGCACTATGC CGTTCTTCTC 
 5401 ATGTATATAT ATATACAGGC AACACGCAGA TATAGGTGCG ACGTGAACAG TGAGCTGTAT 
 5461 GTGCGCAGCT CGCGTTGCAT TTTCGGAAGC GCTCGTTTTC GGAAACGCTT TGAAGTTCCT 
 5521 ATTCCGAAGT TCCTATTCTC TAGCTAGAAA GTATAGGAAC TTCAGAGCGC TTTTGAAAAC 
 5581 CAAAAGCGCT CTGAAGACGC ACTTTCAAAA AACCAAAAAC GCACCGGACT GTAACGAGCT 
 5641 ACTAAAATAT TGCGAATACC GCTTCCACAA ACATTGCTCA AAAGTATCTC TTTGCTATAT 
 5701 ATCTCTGTGC TATATCCCTA TATAACCTAC CCATCCACCT TTCGCTCCTT GAACTTGCAT 
 5761 CTAAACTCGA CCTCTACATT TTTTATGTTT ATCTCTAGTA TTACTCTTTA GACAAAAAAA 
 5821 TTGTAGTAAG AACTATTCAT AGAGTGAATC GAAAACAATA CGAAAATGTA AACATTTCCT 
 5881 ATACGTAGTA TATAGAGACA AAATAGAAGA AACCGTTCAT AATTTTCTGA CCAATGAAGA 
 5941 ATCATCAACG CTATCACTTT CTGTTCACAA AGTATGCGCA ATCCACATCG GTATAGAATA 
 6001 TAATCGGGGA TGCCTTTATC TTGAAAAAAT GCACCCGCAG CTTCGCTAGT AATCAGTAAA 
 6061 CGCGGGAAGT GGAGTCAGGC TTTTTTTATG GAAGAGAAAA TAGACACCAA AGTAGCCTTC 
 6121 TTCTAACCTT AACGGACCTA CAGTGCAAAA AGTTATCAAG AGACTGCATT ATAGAGCGCA 
 6181 CAAAGGAGAA AAAAAGTAAT CTAAGATGCT TTGTTAGAAA AATAGCGCTC TCGGGATGCA 
 6241 TTTTTGTAGA ACAAAAAAGA AGTATAGATT CTTTGTTGGT AAAATAGCGC TCTCGCGTTG 
 6301 CATTTCTGTT CTGTAAAAAT GCAGCTCAGA TTCTTTGTTT GAAAAATTAG CGCTCTCGCG 
 6361 TTGCATTTTT GTTTTACAAA AATGAAGCAC AGATTCTTCG TTGGTAAAAT AGCGCTTTCG 
 6421 CGTTGCATTT CTGTTCTGTA AAAATGCAGC TCAGATTCTT TGTTTGAAAA ATTAGCGCTC 
 6481 TCGCGTTGCA TTTTTGTTCT ACAAAATGAA GCACAGATGC TTCGTT
//