LOCUS       pGAD T7    7988 bp    DNA    SYN    23-Nov-2013
DEFINITION  pGAD T7
ACCESSION    
KEYWORDS    
SOURCE    
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7988
                     /organism="pGAD T7"
                     /mol_type="other DNA"
     promoter        1079..1476
                     /label="yADH1_promoter"
     misc_feature    1501..1557
                     /label="SV40_NLS"
     misc_feature    1492..1899
                     /label="Gal4_AD"
     misc_feature    1561..1899
                     /label="Gal4_AD"
     misc_feature    1825..1842
                     /label="Gal4_AD_primer"
     promoter        1905..1923
                     /label="T7_promoter"
     misc_feature    complement(2295..2318)
                     /label="pJG4_5_rev_primer"
     terminator      2279..2605
                     /label="yADH2_terminator"
     misc_feature    complement(2471..2495)
                     /label="pHybLex_rev_primer"
     terminator      2416..2595
                     /label="yADH1_terminator"
     CDS             complement(2720..3814)
                     /label="LEU2(variant)"
                     /gene="LEU2(variant)"
                     /note="ORF frame 2"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH
                     LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL
                     YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE
                     QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK
                     VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD
                     KNTAFGLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD
                     AGIRTGDLGGSNSTTEVGDAVAEEVKKILA*"
     gene            complement(2720..3814)
                     /label="LEU2(variant)"
                     /gene="LEU2(variant)"
     misc_feature    complement(4258..4276)
                     /label="M13_reverse_primer"
     misc_feature    complement(4275..4297)
                     /label="M13_pUC_rev_primer"
     promoter        complement(4311..4340)
                     /label="lac_promoter"
     misc_feature    complement(4431..4464)
                     /label="loxP"
     misc_feature    complement(4542..4575)
                     /label="loxP"
     rep_origin      complement(4800..5419)
                     /label="pBR322_origin"
     CDS             complement(5574..6434)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /note="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     gene            complement(5574..6434)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     promoter        complement(6476..6504)
                     /label="AmpR_promoter"
     misc_feature    complement(6663..6685)
                     /label="pGEX_3_primer"
     rep_origin      6821..7985
                     /label="2micron2_origin"
ORIGIN
    1 TGCATGCCTG CAGGTCGAGA TCCGGGATCG AAGAAATGAT GGTAAATGAA ATAGGAAATC 
   61 AAGGAGCATG AAGGCAAAAG ACAAATATAA GGGTCGAACG AAAAATAAAG TGAAAAGTGT 
  121 TGATATGATG TATTTGGCTT TGCGGCGCCG AAAAAACGAG TTTACGCAAT TGCACAATCA 
  181 TGCTGACTCT GTGGCGGACC CGCGCTCTTG CCGGCCCGGC GATAACGCTG GGCGTGAGGC 
  241 TGTGCCCGGC GGAGTTTTTT GCGCCTGCAT TTTCCAAGGT TTACCCTGCG CTAAGGGGCG 
  301 AGATTGGAGA AGCAATAAGA ATGCCGGTTG GGGTTGCGAT GATGACGACC ACGACAACTG 
  361 GTGTCATTAT TTAAGTTGCC GAAAGAACCT GAGTGCATTT GCAACATGAG TATACTAGAA 
  421 GAATGAGCCA AGACTTGCGA GACGCGAGTT TGCCGGTGGT GCGAACAATA GAGCGACCAT 
  481 GACCTTGAAG GTGAGACGCG CATAACCGCT AGAGTACTTT GAAGAGGAAA CAGCAATAGG 
  541 GTTGCTACCA GTATAAATAG ACAGGTACAT ACAACACTGG AAATGGTTGT CTGTTTGAGT 
  601 ACGCTTTCAA TTCATTTGGG TGTGCACTTT ATTATGTTAC AATATGGAAG GGAACTTTAC 
  661 ACTTCTCCTA TGCACATATA TTAATTAAAG TCCAATGCTA GTAGAGAAGG GGGGTAACAC 
  721 CCCTCCGCGC TCTTTTCCGA TTTTTTTCTA AACCGTGGAA TATTTCGGAT ATCCTTTTGT 
  781 TGTTTCCGGG TGTACAATAT GGACTTCCTC TTTTCTGGCA ACCAAACCCA TACATCGGGA 
  841 TTCCTATAAT ACCTTCGTTG GTCTCCCTAA CATGTAGGTG GCGGAGGGGA GATATACAAT 
  901 AGAACAGATA CCAGACAAGA CATAATGGGC TAAACAAGAC TACACCAATT ACACTGCCTC 
  961 ATTGATGGTG GTACATAACG AACTAATACT GTAGCCCTAG ACTTGATAGC CATCATCATA 
 1021 TCGAAGTTTC ACTACCCTTT TTCCATTTGC CATCTATTGA AGTAATAATA GGCGCATGCA 
 1081 ACTTCTTTTC TTTTTTTTTC TTTTCTCTCT CCCCCGTTGT TGTCTCACCA TATCCGCAAT 
 1141 GACAAAAAAA ATGATGGAAG ACACTAAAGG AAAAAATTAA CGACAAAGAC AGCACCAACA 
 1201 GATGTCGTTG TTCCAGAGCT GATGAGGGGT ATCTCGAAGC ACACGAAACT TTTTCCTTCC 
 1261 TTCATTCACG CACACTACTC TCTAATGAGC AACGGTATAC GGCCTTCCTT CCAGTTACTT 
 1321 GAATTTGAAA TAAAAAAAAG TTTGCTGTCT TGCTATCAAG TATAAATAGA CCTGCAATTA 
 1381 TTAATCTTTT GTTTCCTCGT CATTGTTCTC GTTCCCTTTC TTCCTTGTTT CTTTTTCTGC 
 1441 ACAATATTTC AAGCTATACC AAGCATACAA TCAACTCCAA GCTTTGCAAA GATGGATAAA 
 1501 GCGGAATTAA TTCCCGAGCC TCCAAAAAAG AAGAGAAAGG TCGAATTGGG TACCGCCGCC 
 1561 AATTTTAATC AAAGTGGGAA TATTGCTGAT AGCTCATTGT CCTTCACTTT CACTAACAGT 
 1621 AGCAACGGTC CGAACCTCAT AACAACTCAA ACAAATTCTC AAGCGCTTTC ACAACCAATT 
 1681 GCCTCCTCTA ACGTTCATGA TAACTTCATG AATAATGAAA TCACGGCTAG TAAAATTGAT 
 1741 GATGGTAATA ATTCAAAACC ACTGTCACCT GGTTGGACGG ACCAAACTGC GTATAACGCG 
 1801 TTTGGAATCA CTACAGGGAT GTTTAATACC ACTACAATGG ATGATGTATA TAACTATCTA 
 1861 TTCGATGATG AAGATACCCC ACCAAACCCA AAAAAAGAGA TCTTTAATAC GACTCACTAT 
 1921 AGGGCGAGCG CCGCCATGGA GTACCCATAC GACGTACCAG ATTACGCTCA TATGGCCATG 
 1981 GAGGCCAGTG AATTCCACCC GGGTGGGCAT CGATACGGGA TCCATCGAGC TCGAGCTGCA 
 2041 GATGAATCGT AGATACTGAA AAACCCCGCA AGTTCACTTC AACTGTGCAT CGTGCACCAT 
 2101 CTCAATTTCT TTCATTTATA CATCGTTTTG CCTTCTTTTA TGTAACTATA CTCCTCTAAG 
 2161 TTTCAATCTT GGCCATGTAA CCTCTGATCT ATAGAATTTT TTAAATGACT AGAATTAATG 
 2221 CCCATCTTTT TTTTGGACCT AAATTCTTCA TGAAAATATA TTACGAGGGC TTATTCAGAA 
 2281 GCTTTGGACT TCTTCGCCAG AGGTTTGGTC AAGTCTCCAA TCAAGGTTGT CGGCTTGTCT 
 2341 ACCTTGCCAG AAATTTACGA AAAGATGGAA AAGGGTCAAA TCGTTGGTAG ATACGTTGTT 
 2401 GACACTTCTA AATAAGCGAA TTTCTTATGA TTTATGATTT TTATTATTAA ATAAGTTATA 
 2461 AAAAAAATAA GTGTATACAA ATTTTAAAGT GACTCTTAGG TTTTAAAACG AAAATTCTTA 
 2521 TTCTTGAGTA ACTCTTTCCT GTAGGTCAGG TTGCTTTCTC AGGTATAGCA TGAGGTCGCT 
 2581 CTTATTGACC ACACCTCTAC CGGCCGGTCG AAATTCCCCT ACCCTATGAA CATATTCCAT 
 2641 TTTGTAATTT CGTGTCGTTT CTATTATGAA TTTCATTTAT AAAGTTTATG TACAAATATC 
 2701 ATAAAAAAAG AGAATCTTTT TAAGCAAGGA TTTTCTTAAC TTCTTCGGCG ACAGCATCAC 
 2761 CGACTTCGGT GGTACTGTTG GAACCACCTA AATCACCAGT TCTGATACCT GCATCCAAAA 
 2821 CCTTTTTAAC TGCATCTTCA ATGGCCTTAC CTTCTTCAGG CAAGTTCAAT GACAATTTCA 
 2881 ACATCATTGC AGCAGACAAG ATAGTGGCGA TAGGGTTGAC CTTATTCTTT GGCAAATCTG 
 2941 GAGCAGAACC GTGGCATGGT TCGTACAAAC CAAATGCGGT GTTCTTGTCT GGCAAAGAGG 
 3001 CCAAGGACGC AGATGGCAAC AAACCCAAGG AACCTGGGAT AACGGAGGCT TCATCGGAGA 
 3061 TGATATCACC AAACATGTTG CTGGTGATTA TAATACCATT TAGGTGGGTT GGGTTCTTAA 
 3121 CTAGGATCAT GGCGGCAGAA TCAATCAATT GATGTTGAAC CTTCAATGTA GGAAATTCGT 
 3181 TCTTGATGGT TTCCTCCACA GTTTTTCTCC ATAATCTTGA AGAGGCCAAA ACATTAGCTT 
 3241 TATCCAAGGA CCAAATAGGC AATGGTGGCT CATGTTGTAG GGCCATGAAA GCGGCCATTC 
 3301 TTGTGATTCT TTGCACTTCT GGAACGGTGT ATTGTTCACT ATCCCAAGCG ACACCATCAC 
 3361 CATCGTCTTC CTTTCTCTTA CCAAAGTAAA TACCTCCCAC TAATTCTCTG ACAACAACGA 
 3421 AGTCAGTACC TTTAGCAAAT TGTGGCTTGA TTGGAGATAA GTCTAAAAGA GAGTCGGATG 
 3481 CAAAGTTACA TGGTCTTAAG TTGGCGTACA ATTGAAGTTC TTTACGGATT TTTAGTAAAC 
 3541 CTTGTTCAGG TCTAACACTA CCTGTACCCC ATTTAGGACC ACCCACAGCA CCTAACAAAA 
 3601 CGGCATCAAC CTTCTTGGAG GCTTCCAGCG CCTCATCTGG AAGTGGGACA CCTGTAGCGT 
 3661 CGATAGCAGC ACCACCAATT AAATGATTTT CGAAATCGAA CTTGACATTG GAACGAACAT 
 3721 CAGAAATAGC TTTAAGAACC TTAATGGCTT CGGCTGTGAT TTCTTGACCA ACGTGGTCAC 
 3781 CTGGCAAAAC GACGATCTTC TTAGGGGCAG ACATTAGAAT GGTATATCCT TGAAATATAT 
 3841 ATATATATTG CTGAAATGTA AAAGGTAAGA AAAGTTAGAA AGTAAGACGA TTGCTAACCA 
 3901 CCTATTGGAA AAAACAATAG GTCCTTAAAT AATATTGTCA ACTTCAAGTA TTGTGATGCA 
 3961 AGCATTTAGT CATGAACGCT TCTCTATTCT ATATGAAAAG CCGGTTCCGG CGCTCTCACC 
 4021 TTTCCTTTTT CTCCCAATTT TTCAGTTGAA AAAGGTATAT GCGTCAGGCG ACCTCTGAAA 
 4081 TTAACAAAAA ATTTCCAGTC ATCGAATTTG ATTCTGTGCG ATAGCGCCCC TGTGTGTTCT 
 4141 CGTTATGTTG AGGAAAAAAA TAATGGTTGC TAAGAGATTC GAACTCTTGC ATCTTACGAT 
 4201 ACCTGAGTAT TCCCACAGTT GGGGATCTCG ACTCTAGCTA GAGGATCAAT TCGTAATCAT 
 4261 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG 
 4321 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG 
 4381 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG ATAACTTCGT 
 4441 ATAATGTATG CTATACGAAG TTATTAGGTC TGAAGAGGAG TTTACGTCCA GCCAAGCTAG 
 4501 CTTGGCTGCA GGTCGAGCGG CCGCGATCCG GAACCCTTAA TATAACTTCG TATAATGTAT 
 4561 GCTATACGAA GTTATCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG 
 4621 CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG 
 4681 CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT 
 4741 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC 
 4801 GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC 
 4861 TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA 
 4921 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT 
 4981 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG 
 5041 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC 
 5101 GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG 
 5161 GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC 
 5221 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG 
 5281 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC 
 5341 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT 
 5401 CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT 
 5461 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA 
 5521 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA 
 5581 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC 
 5641 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT 
 5701 GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA 
 5761 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT 
 5821 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT 
 5881 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC 
 5941 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC 
 6001 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT 
 6061 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT 
 6121 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC 
 6181 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT 
 6241 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG 
 6301 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT 
 6361 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA 
 6421 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
 6481 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
 6541 ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC 
 6601 TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC GTTTCGGTGA TGACGGTGAA 
 6661 AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG 
 6721 AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CTGGCTTAAC 
 6781 TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA ACGCATTTAA GCATAAACAC 
 6841 GCACTATGCC GTTCTTCTCA TGTATATATA TATACAGGCA ACACGCAGAT ATAGGTGCGA 
 6901 CGTGAACAGT GAGCTGTATG TGCGCAGCTC GCGTTGCATT TTCGGAAGCG CTCGTTTTCG 
 6961 GAAACGCTTT GAAGTTCCTA TTCCGAAGTT CCTATTCTCT AGCTAGAAAG TATAGGAACT 
 7021 TCAGAGCGCT TTTGAAAACC AAAAGCGCTC TGAAGACGCA CTTTCAAAAA ACCAAAAACG 
 7081 CACCGGACTG TAACGAGCTA CTAAAATATT GCGAATACCG CTTCCACAAA CATTGCTCAA 
 7141 AAGTATCTCT TTGCTATATA TCTCTGTGCT ATATCCCTAT ATAACCTACC CATCCACCTT 
 7201 TCGCTCCTTG AACTTGCATC TAAACTCGAC CTCTACATTT TTTATGTTTA TCTCTAGTAT 
 7261 TACTCTTTAG ACAAAAAAAT TGTAGTAAGA ACTATTCATA GAGTGAATCG AAAACAATAC 
 7321 GAAAATGTAA ACATTTCCTA TACGTAGTAT ATAGAGACAA AATAGAAGAA ACCGTTCATA 
 7381 ATTTTCTGAC CAATGAAGAA TCATCAACGC TATCACTTTC TGTTCACAAA GTATGCGCAA 
 7441 TCCACATCGG TATAGAATAT AATCGGGGAT GCCTTTATCT TGAAAAAATG CACCCGCAGC 
 7501 TTCGCTAGTA ATCAGTAAAC GCGGGAAGTG GAGTCAGGCT TTTTTTATGG AAGAGAAAAT 
 7561 AGACACCAAA GTAGCCTTCT TCTAACCTTA ACGGACCTAC AGTGCAAAAA GTTATCAAGA 
 7621 GACTGCATTA TAGAGCGCAC AAAGGAGAAA AAAAGTAATC TAAGATGCTT TGTTAGAAAA 
 7681 ATAGCGCTCT CGGGATGCAT TTTTGTAGAA CAAAAAAGAA GTATAGATTC TTTGTTGGTA 
 7741 AAATAGCGCT CTCGCGTTGC ATTTCTGTTC TGTAAAAATG CAGCTCAGAT TCTTTGTTTG 
 7801 AAAAATTAGC GCTCTCGCGT TGCATTTTTG TTTTACAAAA ATGAAGCACA GATTCTTCGT 
 7861 TGGTAAAATA GCGCTTTCGC GTTGCATTTC TGTTCTGTAA AAATGCAGCT CAGATTCTTT 
 7921 GTTTGAAAAA TTAGCGCTCT CGCGTTGCAT TTTTGTTCTA CAAAATGAAG CACAGATGCT 
 7981 TCGTTGCT
//