LOCUS       pPIC9K	9276 bp 	DNA   circular	SYN
DEFINITION  pPIC9K
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..9276
                     /organism="pPIC9K"
                     /mol_type="other DNA"
     misc_feature    1152..1172
                     /label="alpha_factor_primer"
     misc_feature    complement(1327..1347)
                     /label="3_AOX1_primer"
     CDS             complement(1980..4514)
                     /label="ORF frame 2"
     gene            complement(4928..5743)
                     /label="KanR2"
                     /gene="KanR2"
     CDS             complement(4928..5743)
                     /label="ORF frame 3"
     misc_feature    complement(5908..5927)
                     /label="pBRrevBam_primer"
     CDS             complement(5909..6631)
                     /label="ORF frame 3"
     misc_feature    complement(6913..6935)
                     /label="pGEX_3_primer"
     rep_origin      complement(7332..7951)
                     /label="pBR322_origin"
     gene            complement(8106..8966)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(8106..8966)
                     /label="ORF frame 2"
     promoter        complement(9008..9036)
                     /label="AmpR_promoter"
ORIGIN
    1 AGATCTAACA TCCAAAGACG AAAGGTTGAA TGAAACCTTT TTGCCATCCG ACATCCACAG
   61 GTCCATTCTC ACACATAAGT GCCAAACGCA ACAGGAGGGG ATACACTAGC AGCAGACCGT
  121 TGCAAACGCA GGACCTCCAC TCCTCTTCTC CTCAACACCC ACTTTTGCCA TCGAAAAACC
  181 AGCCCAGTTA TTGGGCTTGA TTGGAGCTCG CTCATTCCAA TTCCTTCTAT TAGGCTACTA
  241 ACACCATGAC TTTATTAGCC TGTCTATCCT GGCCCCCCTG GCGAGGTTCA TGTTTGTTTA
  301 TTTCCGAATG CAACAAGCTC CGCATTACAC CCGAACATCA CTCCAGATGA GGGCTTTCTG
  361 AGTGTGGGGT CAAATAGTTT CATGTTCCCC AAATGGCCCA AAACTGACAG TTTAAACGCT
  421 GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG
  481 TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGCCA TACCGTTTGT
  541 CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT
  601 CTCTATCGCT TCTGAACCCC GGTGCACCTG TGCCGAAACG CAAATGGGGA AACACCCGCT
  661 TTTTGGATGA TTATGCATTG TCTCCACATT GTATGCTTCC AAGATTCTGG TGGGAATACT
  721 GCTGATAGCC TAACGTTCAT GATCAAAATT TAACTGTTCT AACCCCTACT TGACAGCAAT
  781 ATATAAACAG AAGGAAGCTG CCCTGTCTTA AACCTTTTTT TTTATCATCA TTATTAGCTT
  841 ACTTTCATAA TTGCGACTGG TTCCAATTGA CAAGCTTTTG ATTTTAACGA CTTTTAACGA
  901 CAACTTGAGA AGATCAAAAA ACAACTAATT ATTCGAAGGA TCCAAACGAT GAGATTTCCT
  961 TCAATTTTTA CTGCAGTTTT ATTCGCAGCA TCCTCCGCAT TAGCTGCTCC AGTCAACACT
 1021 ACAACAGAAG ATGAAACGGC ACAAATTCCG GCTGAAGCTG TCATCGGTTA CTCAGATTTA
 1081 GAAGGGGATT TCGATGTTGC TGTTTTGCCA TTTTCCAACA GCACAAATAA CGGGTTATTG
 1141 TTTATAAATA CTACTATTGC CAGCATTGCT GCTAAAGAAG AAGGGGTATC TCTCGAGAAA
 1201 AGAGAGGCTG AAGCTTACGT AGAATTCCCT AGGGCGGCCG CGAATTAATT CGCCTTAGAC
 1261 ATGACTGTTC CTCAGTTCAA GTTGGGCACT TACGAGAAGA CCGGTCTTGC TAGATTCTAA
 1321 TCAAGAGGAT GTCAGAATGC CATTTGCCTG AGAGATGCAG GCTTCATTTT TGATACTTTT
 1381 TTATTTGTAA CCTATATAGT ATAGGATTTT TTTTGTCATT TTGTTTCTTC TCGTACGAGC
 1441 TTGCTCCTGA TCAGCCTATC TCGCAGCTGA TGAATATCTT GTGGTAGGGG TTTGGGAAAA
 1501 TCATTCGAGT TTGATGTTTT TCTTGGTATT TCCCACTCCT CTTCAGAGTA CAGAAGATTA
 1561 AGTGAGAAGT TCGTTTGTGC AAGCTTATCG ATAAGCTTTA ATGCGGTAGT TTATCACAGT
 1621 TAAATTGCTA ACGCAGTCAG GCACCGTGTA TGAAATCTAA CAATGCGCTC ATCGTCATCC
 1681 TCGGCACCGT CACCCTGGAT GCTGTAGGCA TAGGCTTGGT TATGCCGGTA CTGCCGGGCC
 1741 TCTTGCGGGA TATCGTCCAT TCCGACAGCA TCGCCAGTCA CTATGGCGTG CTGCTAGCGC
 1801 TATATGCGTT GATGCAATTT CTATGCGCAC CCGTTCTCGG AGCACTGTCC GACCGCTTTG
 1861 GCCGCCGCCC AGTCCTGCTC GCTTCGCTAC TTGGAGCCAC TATCGACTAC GCGATCATGG
 1921 CGACCACACC CGTCCTGTGG ATCTATCGAA TCTAAATGTA AGTTAAAATC TCTAAATAAT
 1981 TAAATAAGTC CCAGTTTCTC CATACGAACC TTAACAGCAT TGCGGTGAGC ATCTAGACCT
 2041 TCAACAGCAG CCAGATCCAT CACTGCTTGG CCAATATGTT TCAGTCCCTC AGGAGTTACG
 2101 TCTTGTGAAG TGATGAACTT CTGGAAGGTT GCAGTGTTAA CTCCGCTGTA TTGACGGGCA
 2161 TATCCGTACG TTGGCAAAGT GTGGTTGGTA CCGGAGGAGT AATCTCCACA ACTCTCTGGA
 2221 GAGTAGGCAC CAACAAACAC AGATCCAGCG TGTTGTACTT GATCAACATA AGAAGAAGCA
 2281 TTCTCGATTT GCAGGATCAA GTGTTCAGGA GCGTACTGAT TGGACATTTC CAAAGCCTGC
 2341 TCGTAGGTTG CAACCGATAG GGTTGTAGAG TGTGCAATAC ACTTGCGTAC AATTTCAACC
 2401 CTTGGCAACT GCACAGCTTG GTTGTGAACA GCATCTTCAA TTCTGGCAAG CTCCTTGTCT
 2461 GTCATATCGA CAGCCAACAG AATCACCTGG GAATCAATAC CATGTTCAGC TTGAGACAGA
 2521 AGGTCTGAGG CAACGAAATC TGGATCAGCG TATTTATCAG CAATAACTAG AACTTCAGAA
 2581 GGCCCAGCAG GCATGTCAAT ACTACACAGG GCTGATGTGT CATTTTGAAC CATCATCTTG
 2641 GCAGCAGTAA CGAACTGGTT TCCTGGACCA AATATTTTGT CACACTTAGG AACAGTTTCT
 2701 GTTCCGTAAG CCATAGCAGC TACTGCCTGG GCGCCTCCTG CTAGCACGAT ACACTTAGCA
 2761 CCAACCTTGT GGGCAACGTA GATGACTTCT GGGGTAAGGG TACCATCCTT CTTAGGTGGA
 2821 GATGCAAAAA CAATTTCTTT GCAACCAGCA ACTTTGGCAG GAACACCCAG CATCAGGGAA
 2881 GTGGAAGGCA GAATTGCGGT TCCACCAGGA ATATAGAGGC CAACTTTCTC AATAGGTCTT
 2941 GCAAAACGAG AGCAGACTAC ACCAGGGCAA GTCTCAACTT GCAACGTCTC CGTTAGTTGA
 3001 GCTTCATGGA ATTTCCTGAC GTTATCTATA GAGAGATCAA TGGCTCTCTT AACGTTATCT
 3061 GGCAATTGCA TAAGTTCCTC TGGGAAAGGA GCTTCTAACA CAGGTGTCTT CAAAGCGACT
 3121 CCATCAAACT TGGCAGTTAG TTCTAAAAGG GCTTTGTCAC CATTTTGACG AACATTGTCG
 3181 ACAATTGGTT TGACTAATTC CATAATCTGT TCCGTTTTCT GGATAGGACG ACGAAGGGCA
 3241 TCTTCAATTT CTTGTGAGGA GGCCTTAGAA ACGTCAATTT TGCACAATTC AATACGACCT
 3301 TCAGAAGGGA CTTCTTTAGG TTTGGATTCT TCTTTAGGTT GTTCCTTGGT GTATCCTGGC
 3361 TTGGCATCTC CTTTCCTTCT AGTGACCTTT AGGGACTTCA TATCCAGGTT TCTCTCCACC
 3421 TCGTCCAACG TCACACCGTA CTTGGCACAT CTAACTAATG CAAAATAAAA TAAGTCAGCA
 3481 CATTCCCAGG CTATATCTTC CTTGGATTTA GCTTCTGCAA GTTCATCAGC TTCCTCCCTA
 3541 ATTTTAGCGT TCAACAAAAC TTCGTCGTCA AATAACCGTT TGGTATAAGA ACCTTCTGGA
 3601 GCATTGCTCT TACGATCCCA CAAGGTGGCT TCCATGGCTC TAAGACCCTT TGATTGGCCA
 3661 AAACAGGAAG TGCGTTCCAA GTGACAGAAA CCAACACCTG TTTGTTCAAC CACAAATTTC
 3721 AAGCAGTCTC CATCACAATC CAATTCGATA CCCAGCAACT TTTGAGTTGC TCCAGATGTA
 3781 GCACCTTTAT ACCACAAACC GTGACGACGA GATTGGTAGA CTCCAGTTTG TGTCCTTATA
 3841 GCCTCCGGAA TAGACTTTTT GGACGAGTAC ACCAGGCCCA ACGAGTAATT AGAAGAGTCA
 3901 GCCACCAAAG TAGTGAATAG ACCATCGGGG CGGTCAGTAG TCAAAGACGC CAACAAAATT
 3961 TCACTGACAG GGAACTTTTT GACATCTTCA GAAAGTTCGT ATTCAGTAGT CAATTGCCGA
 4021 GCATCAATAA TGGGGATTAT ACCAGAAGCA ACAGTGGAAG TCACATCTAC CAACTTTGCG
 4081 GTCTCAGAAA AAGCATAAAC AGTTCTACTA CCGCCATTAG TGAAACTTTT CAAATCGCCC
 4141 AGTGGAGAAG AAAAAGGCAC AGCGATACTA GCATTAGCGG GCAAGGATGC AACTTTATCA
 4201 ACCAGGGTCC TATAGATAAC CCTAGCGCCT GGGATCATCC TTTGGACAAC TCTTTCTGCC
 4261 AAATCTAGGT CCAAAATCAC TTCATTGATA CCATTATTGT ACAACTTGAG CAAGTTGTCG
 4321 ATCAGCTCCT CAAATTGGTC CTCTGTAACG GATGACTCAA CTTGCACATT AACTTGAAGC
 4381 TCAGTCGATT GAGTGAACTT GATCAGGTTG TGCAGCTGGT CAGCAGCATA GGGAAACACG
 4441 GCTTTTCCTA CCAAACTCAA GGAATTATCA AACTCTGCAA CACTTGCGTA TGCAGGTAGC
 4501 AAGGGAAATG TCATACTTGA AGTCGGACAG TGAGTGTAGT CTTGAGAAAT TCTGAAGCCG
 4561 TATTTTTATT ATCAGTGAGT CAGTCATCAG GAGATCCTCT ACGCCGGACG CATCGTGGCC
 4621 GACCTGCAGG GGGGGGGGGG GCGCTGAGGT CTGCCTCGTG AAGAAGGTGT TGCTGACTCA
 4681 TACCAGGCCT GAATCGCCCC ATCATCCAGC CAGAAAGTGA GGGAGCCACG GTTGATGAGA
 4741 GCTTTGTTGT AGGTGGACCA GTTGGTGATT TTGAACTTTT GCTTTGCCAC GGAACGGTCT
 4801 GCGTTGTCGG GAAGATGCGT GATCTGATCC TTCAACTCAG CAAAAGTTCG ATTTATTCAA
 4861 CAAAGCCGCC GTCCCGTCAA GTCAGCGTAA TGCTCTGCCA GTGTTACAAC CAATTAACCA
 4921 ATTCTGATTA GAAAAACTCA TCGAGCATCA AATGAAACTG CAATTTATTC ATATCAGGAT
 4981 TATCAATACC ATATTTTTGA AAAAGCCGTT TCTGTAATGA AGGAGAAAAC TCACCGAGGC
 5041 AGTTCCATAG GATGGCAAGA TCCTGGTATC GGTCTGCGAT TCCGACTCGT CCAACATCAA
 5101 TACAACCTAT TAATTTCCCC TCGTCAAAAA TAAGGTTATC AAGTGAGAAA TCACCATGAG
 5161 TGACGACTGA ATCCGGTGAG AATGGCAAAA GCTTATGCAT TTCTTTCCAG ACTTGTTCAA
 5221 CAGGCCAGCC ATTACGCTCG TCATCAAAAT CACTCGCATC AACCAAACCG TTATTCATTC
 5281 GTGATTGCGC CTGAGCGAGA CGAAATACGC GATCGCTGTT AAAAGGACAA TTACAAACAG
 5341 GAATCGAATG CAACCGGCGC AGGAACACTG CCAGCGCATC AACAATATTT TCACCTGAAT
 5401 CAGGATATTC TTCTAATACC TGGAATGCTG TTTTCCCGGG GATCGCAGTG GTGAGTAACC
 5461 ATGCATCATC AGGAGTACGG ATAAAATGCT TGATGGTCGG AAGAGGCATA AATTCCGTCA
 5521 GCCAGTTTAG TCTGACCATC TCATCTGTAA CATCATTGGC AACGCTACCT TTGCCATGTT
 5581 TCAGAAACAA CTCTGGCGCA TCGGGCTTCC CATACAATCG ATAGATTGTC GCACCTGATT
 5641 GCCCGACATT ATCGCGAGCC CATTTATACC CATATAAATC AGCATCCATG TTGGAATTTA
 5701 ATCGCGGCCT CGAGCAAGAC GTTTCCCGTT GAATATGGCT CATAACACCC CTTGTATTAC
 5761 TGTTTATGTA AGCAGACAGT TTTATTGTTC ATGATGATAT ATTTTTATCT TGTGCAATGT
 5821 AACATCAGAG ATTTTGAGAC ACAACGTGGC TTTCCCCCCC CCCCCTGCAG GTCGGCATCA
 5881 CCGGCGCCAC AGGTGCGGTT GCTGGCGCCT ATATCGCCGA CATCACCGAT GGGGAAGATC
 5941 GGGCTCGCCA CTTCGGGCTC ATGAGCGCTT GTTTCGGCGT GGGTATGGTG GCAGGCCCCG
 6001 TGGCCGGGGG ACTGTTGGGC GCCATCTCCT TGCATGCACC ATTCCTTGCG GCGGCGGTGC
 6061 TCAACGGCCT CAACCTACTA CTGGGCTGCT TCCTAATGCA GGAGTCGCAT AAGGGAGAGC
 6121 GTCGAGTATC TATGATTGGA AGTATGGGAA TGGTGATACC CGCATTCTTC AGTGTCTTGA
 6181 GGTCTCCTAT CAGATTATGC CCAACTAAAG CAACCGGAGG AGGAGATTTC ATGGTAAATT
 6241 TCTCTGACTT TTGGTCATCA GTAGACTCGA ACTGTGAGAC TATCTCGGTT ATGACAGCAG
 6301 AAATGTCCTT CTTGGAGACA GTAAATGAAG TCCCACCAAT AAAGAAATCC TTGTTATCAG
 6361 GAACAAACTT CTTGTTTCGA ACTTTTTCGG TGCCTTGAAC TATAAAATGT AGAGTGGATA
 6421 TGTCGGGTAG GAATGGAGCG GGCAAATGCT TACCTTCTGG ACCTTCAAGA GGTATGTAGG
 6481 GTTTGTAGAT ACTGATGCCA ACTTCAGTGA CAACGTTGCT ATTTCGTTCA AACCATTCCG
 6541 AATCCAGAGA AATCAAAGTT GTTTGTCTAC TATTGATCCA AGCCAGTGCG GTCTTGAAAC
 6601 TGACAATAGT GTGCTCGTGT TTTGAGGTCA TCTTTGTATG AATAAATCTA GTCTTTGATC
 6661 TAAATAATCT TGACGAGCCA AGGCGATAAA TACCCAAATC TAAAACTCTT TTAAAACGTT
 6721 AAAAGGACAA GTATGTCTGC CTGTATTAAA CCCCAAATCA GCTCGTAGTC TGATCCTCAT
 6781 CAACTTGAGG GGCACTATCT TGTTTTAGAG AAATTTGCGG AGATGCGATA TCGAGAAAAA
 6841 GGTACGCTGA TTTTAAACGT GAAATTTATC TCAAGATCTC TGCCTCGCGC GTTTCGGTGA
 6901 TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC
 6961 GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG
 7021 CGCAGCCATG ACCCAGTCAC GTAGCGATAG CGGAGTGTAT ACTGGCTTAA CTATGCGGCA
 7081 TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA
 7141 AGGAGAAAAT ACCGCATCAG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG
 7201 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA
 7261 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
 7321 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC
 7381 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
 7441 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC
 7501 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT
 7561 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
 7621 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC
 7681 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
 7741 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT
 7801 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC
 7861 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA
 7921 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC
 7981 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
 8041 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT
 8101 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA
 8161 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
 8221 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA
 8281 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
 8341 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG
 8401 CGCAACGTTG TTGCCATTGC TGCAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT
 8461 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
 8521 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA
 8581 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC
 8641 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG
 8701 AGTTGCTCTT GCCCGGCGTC AACACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA
 8761 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
 8821 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC
 8881 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG
 8941 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT
 9001 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA
 9061 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC
 9121 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTTCA AGAATTAATT
 9181 CTCATGTTTG ACAGCTTATC ATCGATAAGC TGACTCATGT TGGTATTGTG AAATAGACGC
 9241 AGATCGGGAA CACTGAAAAA TAACAGTTAT TATTCG
//