NOTE: The attached sequence file has been compiled from information in the sequence databases,published literature, and other sources, together with partial sequences obtained by Clontech. This vector has not been completely sequenced.



LOCUS       pBacPAK8	5538 bp 	DNA	SYN
DEFINITION  pBacPAK8
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..5538
                     /organism="pBacPAK8"
                     /mol_type="other DNA"
     misc_feature    1..1147
                     /label="Ac_5_flank"
     CDS             complement(489..1094)
                     /label="ORF frame 2"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     promoter        1148..1248
                     /label="PH_promoter"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1174..1191
                     /label="polyhedrin_fwd_primer"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     misc_feature    1337..2770
                     /label="Ac_3_flank"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNG
                     VIELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIH
                     PIKKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKII
                     LSQINCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR*"
     CDS             complement(1353..2606)
                     /label="ORF frame 2"
                     /translation="MLIADFYDLYSNYNIELPPPQALPRSRRPSVVQPAAPAPVPTIV
                     REQTKPEQIIPAAPPPPPSPVPNIPAPPPPPPPSMSELPPAPPMPTEPQPAAPLDDRQ
                     QLLEAIRNEKNRTRLRPVKPKTAPETSTIVEVPTVLPKETFEPKPPSASPPPPPPPPP
                     PPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPPAPSLSNVLSELKSGTVRLKPAQKRP
                     QSEIIPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNRPNKANTPDV
                     KYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKARLLLQDLAS
                     FVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIFALTYHPK
                     DYKFNELLKYVQQLSVNQQRTESSA*"
     misc_feature    complement(1435..2233)
                     /label="r_ORF1629"
                     /translation="MLIADFYDLYSNYNIELPPPQALPRSRRPSVVQPAAPAPVPTIV
                     REQTKPEQIIPAAPPPPPSPVPNIPAPPPPPPPSMSELPPAPPMPTEPQPAAPLDDRQ
                     QLLEAIRNEKNRTRLRPVKPKTAPETSTIVEVPTVLPKETFEPKPPSASPPPPPPPPP
                     PPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPPAPSLSNVLSELKSGTVRLKPAQKRP
                     QSEIIPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNRPNKANTPDV
                     KYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKARLLLQDLAS
                     FVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIFALTYHPK
                     DYKFNELLKYVQQLSVNQQRTESSA*"
     rep_origin      2858..3164
                     /label="f1_origin"
                     /translation="MLIADFYDLYSNYNIELPPPQALPRSRRPSVVQPAAPAPVPTIV
                     REQTKPEQIIPAAPPPPPSPVPNIPAPPPPPPPSMSELPPAPPMPTEPQPAAPLDDRQ
                     QLLEAIRNEKNRTRLRPVKPKTAPETSTIVEVPTVLPKETFEPKPPSASPPPPPPPPP
                     PPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPPAPSLSNVLSELKSGTVRLKPAQKRP
                     QSEIIPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNRPNKANTPDV
                     KYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKARLLLQDLAS
                     FVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIFALTYHPK
                     DYKFNELLKYVQQLSVNQQRTESSA*"
     misc_feature    3432..3454
                     /label="pGEX_3_primer"
                     /translation="MLIADFYDLYSNYNIELPPPQALPRSRRPSVVQPAAPAPVPTIV
                     REQTKPEQIIPAAPPPPPSPVPNIPAPPPPPPPSMSELPPAPPMPTEPQPAAPLDDRQ
                     QLLEAIRNEKNRTRLRPVKPKTAPETSTIVEVPTVLPKETFEPKPPSASPPPPPPPPP
                     PPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPPAPSLSNVLSELKSGTVRLKPAQKRP
                     QSEIIPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNRPNKANTPDV
                     KYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKARLLLQDLAS
                     FVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIFALTYHPK
                     DYKFNELLKYVQQLSVNQQRTESSA*"
     promoter        3613..3641
                     /label="AmpR_promoter"
                     /translation="MLIADFYDLYSNYNIELPPPQALPRSRRPSVVQPAAPAPVPTIV
                     REQTKPEQIIPAAPPPPPSPVPNIPAPPPPPPPSMSELPPAPPMPTEPQPAAPLDDRQ
                     QLLEAIRNEKNRTRLRPVKPKTAPETSTIVEVPTVLPKETFEPKPPSASPPPPPPPPP
                     PPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPPAPSLSNVLSELKSGTVRLKPAQKRP
                     QSEIIPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNRPNKANTPDV
                     KYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKARLLLQDLAS
                     FVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIFALTYHPK
                     DYKFNELLKYVQQLSVNQQRTESSA*"
     gene            3683..4543
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MLIADFYDLYSNYNIELPPPQALPRSRRPSVVQPAAPAPVPTIV
                     REQTKPEQIIPAAPPPPPSPVPNIPAPPPPPPPSMSELPPAPPMPTEPQPAAPLDDRQ
                     QLLEAIRNEKNRTRLRPVKPKTAPETSTIVEVPTVLPKETFEPKPPSASPPPPPPPPP
                     PPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPPAPSLSNVLSELKSGTVRLKPAQKRP
                     QSEIIPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNRPNKANTPDV
                     KYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKARLLLQDLAS
                     FVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIFALTYHPK
                     DYKFNELLKYVQQLSVNQQRTESSA*"
     CDS             3683..4543
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4698..5317
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag
   61 aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa
  121 caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt
  181 accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt
  241 ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca
  301 cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa
  361 accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg
  421 caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgctaaattt
  481 gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt
  541 gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag
  601 ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct
  661 tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat
  721 ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt
  781 ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt
  841 tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg
  901 ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt
  961 acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta
 1021 aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta
 1081 aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc
 1141 aatatatagt tgctgatatc atggagataa ttaaaatgat aaccatctcg caaataaata
 1201 agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata cggatccctg
 1261 caggcctcga gttcgaatct agaagatctg gtaccgagct cgaattcccg ggcggccgct
 1321 taattaattg atccgggtta ttagtacatt tattaagcgc tagattctgt gcgttgttga
 1381 tttacagaca attgttgtac gtattttaat aattcattaa atttataatc tttagggtgg
 1441 tatgttagag cgaaaatcaa atgattttca gcgtctttat atctgaattt aaatattaaa
 1501 tcctcaatag atttgtaaaa taggtttcga ttagtttcaa acaagggttg tttttccgaa
 1561 ccgatggctg gactatctaa tggattttcg ctcaacgcca caaaacttgc caaatcttgt
 1621 agcagcaatc tagctttgtc gatattcgtt tgtgttttgt tttgtaataa aggttcgacg
 1681 tcgttcaaaa tattatgcgc ttttgtattt ctttcatcac tgtcgttagt gtacaattga
 1741 ctcgacgtaa acacgttaaa taaagcttgg acatatttaa catcgggcgt gttagcttta
 1801 ttaggccgat tatcgtcgtc gtcccaaccc tcgtcgttag aagttgcttc cgaagacgat
 1861 tttgccatag ccacacgacg cctattaatt gtgtcggcta acacgtccgc gatcaaattt
 1921 gtagttgagc tttttggaat tatttctgat tgcgggcgtt tttgggcggg tttcaatcta
 1981 actgtgcccg attttaattc agacaacacg ttagaaagcg atggtgcagg cggtggtaac
 2041 atttcagacg gcaaatctac taatggcggc ggtggtggag ctgatgataa atctaccatc
 2101 ggtggaggcg caggcggggc tggcggcgga ggcggaggcg gaggtggtgg cggtgatgca
 2161 gacggcggtt taggctcaaa tgtctcttta ggcaacacag tcggcacctc aactattgta
 2221 ctggtttcgg gcgccgtttt tggtttgacc ggtctgagac gagtgcgatt tttttcgttt
 2281 ctaatagctt ccaacaattg ttgtctgtcg tctaaaggtg cagcgggttg aggttccgtc
 2341 ggcattggtg gagcgggcgg caattcagac atcgatggtg gtggtggtgg tggaggcgct
 2401 ggaatgttag gcacgggaga aggtggtggc ggcggtgccg ccggtataat ttgttctggt
 2461 ttagtttgtt cgcgcacgat tgtgggcacc ggcgcaggcg ccgctggctg cacaacggaa
 2521 ggtcgtctgc ttcgaggcag cgcttggggt ggtggcaatt caatattata attggaatac
 2581 aaatcgtaaa aatctgctat aagcattgta atttcgctat cgtttaccgt gccgatattt
 2641 aacaaccgct caatgtaagc aattgtattg taaagagatt gtctcaagct cggatcgatc
 2701 ccgcacgccg ataacaagcc ttttcatttt tactacagca ttgtagtggc gagacacttc
 2761 gctgtcgtcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
 2821 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
 2881 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
 2941 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
 3001 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
 3061 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
 3121 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
 3181 gggctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
 3241 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
 3301 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
 3361 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
 3421 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
 3481 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
 3541 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
 3601 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
 3661 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
 3721 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
 3781 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
 3841 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
 3901 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
 3961 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
 4021 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
 4081 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
 4141 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
 4201 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
 4261 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
 4321 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
 4381 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
 4441 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
 4501 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
 4561 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
 4621 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
 4681 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
 4741 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
 4801 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
 4861 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
 4921 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
 4981 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
 5041 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
 5101 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
 5161 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
 5221 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
 5281 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
 5341 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
 5401 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
 5461 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
 5521 gccgattcat taatgcag
//