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LOCUS pIZ/V5-His 2876 bp DNA SYN DEFINITION pIZ/V5-His ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..2876 /organism="pIZ/V5-His" /mol_type="other DNA" misc_feature 515..534 /label="OpIE2_fwd_primer" rep_origin complement(991..1610) /label="pBR322_origin" misc_feature 2176..2195 /label="OpIE2_fwd_primer" promoter 2281..2299 /label="T7_promoter" gene 2309..2680 /label="bleo" /gene="bleo" misc_feature 2309..2683 /label="sh_ble" terminator 2750..5 /label="SV40_PA_terminator" misc_feature 2838..2857 /label="EBV_rev_primer" ORIGIN 1 GGATCATGAT GATAAACAAT GTATGGTGCT AATGTTGCTT CAACAACAAT TCTGTTGAAC 61 TGTGTTTTCA TGTTTGCCAA CAAGCACCTT TATACTCGGT GGCCTCCCCA CCACCAACTT 121 TTTTGCACTG CAAAAAAACA CGCTTTTGCA CGCGGGCCCA TACATAGTAC AAACTCTACG 181 TTTCGTAGAC TATTTTACAT AAATAGTCTA CACCGTTGTA TACGCTCCAA ATACACTACC 241 ACACATTGAA CCTTTTTGCA GTGCAAAAAA GTACGTGTCG GCAGTCACGT AGGCCGGCCT 301 TATCGGGTCG CGTCCTGTCA CGTACGAATC ACATTATCGG ACCGGACGAG TGTTGTCTTA 361 TCGTGACAGG ACGCCAGCTT CCTGTGTTGC TAACCGCAGC CGGACGCAAC TCCTTATCGG 421 AACAGGACGC GCCTCCATAT CAGCCGCGCG TTATCTCATG CGCGTGACCG GACACGAGGC 481 GCCCGTCCCG CTTATCGCGC CTATAAATAC AGCCCGCAAC GATCTGGTAA ACACAGTTGA 541 ACAGCATCTG TTCGAATTTA AAGCTTGGTA CCGAGCTCGG ATCCACTAGT CCAGTGTGGT 601 GGAATTCTGC AGATATCCAG CACAGTGGCG GCCGCTCGAG TCTAGAGGGC CCGCGGTTCG 661 AAGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGCGTACC GGTCATCATC 721 ACCATCACCA TTGAGTTTAT CTGACTAAAT CTTAGTTTGT ATTGTCATGT TTTAATACAA 781 TATGTTATGT TTAAATATGT TTTTAATAAA TTTTATAAAA TAATTTCAAC TTTTATTGTA 841 ACAACATTGT CCATTTACAC ACTCCTTTCA AGCGCGTGGG ATCGATGCTC ACTCAAAGGC 901 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 961 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 1021 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 1081 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 1141 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 1201 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 1261 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 1321 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 1381 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 1441 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 1501 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 1561 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 1621 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA TGATAAACAA 1681 TGTATGGTGC TAATGTTGCT TCAACAACAA TTCTGTTGAA CTGTGTTTTC ATGTTTGCCA 1741 ACAAGCACCT TTATACTCGG TGGCCTCCCC ACCACCAACT TTTTTGCACT GCAAAAAAAC 1801 ACGCTTTTGC ACGCGGGCCC ATACATAGTA CAAACTCTAC GTTTCGTAGA CTATTTTACA 1861 TAAATAGTCT ACACCGTTGT ATACGCTCCA AATACACTAC CACACATTGA ACCTTTTTGC 1921 AGTGCAAAAA AGTACGTGTC GGCAGTCACG TAGGCCGGCC TTATCGGGTC GCGTCCTGTC 1981 ACGTACGAAT CACATTATCG GACCGGACGA GTGTTGTCTT ATCGTGACAG GACGCCAGCT 2041 TCCTGTGTTG CTAACCGCAG CCGGACGCAA CTCCTTATCG GAACAGGACG CGCCTCCATA 2101 TCAGCCGCGC GTTATCTCAT GCGCGTGACC GGACACGAGG CGCCCGTCCC GCTTATCGCG 2161 CCTATAAATA CAGCCCGCAA CGATCTGGTA AACACAGTTG AACAGCATCT GTTCGAATTA 2221 ATTCGGATCT CTGCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA 2281 TAATACGACT CACTATAGGA GGGCCACCAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT 2341 CACCGCGCGC GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG 2401 GGACTTCGTG GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG 2461 CGCGGTCCAG GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT 2521 GGACGAGCTG TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG 2581 GCCGGCCATG ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC 2641 GGCCGGCAAC TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGACCGACGC CGACCAACAC 2701 CGCCGGTCCG ACGGCGGCCC ACGGGTCCCA GGGGGGTCGA CCTCGAAACT TGTTTATTGC 2761 AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT 2821 TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCT //