LOCUS       pIEx/Bac-4   7496 bp    DNA   circular            22-AUG-2007
DEFINITION  pREN4494: pIEx/Bac p10/AAT/Pseudo UTR/GST/EkLIC/10xHis.
SOURCE      
  ORGANISM
FEATURES             Location/Qualifiers
     promoter        1682..2228
                     /vntifkey="29"
                     /label=ie1\Promoter
     misc_recomb     complement(3616..5034)
                     /vntifkey="86"
                     /label=1629
     rep_origin      6971..7426
                     /vntifkey="33"
                     /label=f1\origin
     CDS             complement(5982..6839)
                     /vntifkey="4"
                     /label=Ap
     rep_origin      complement(5221..5221)
                     /vntifkey="33"
                     /label=origin
     misc_recomb     4..453
                     /vntifkey="86"
                     /label=lef-2
     misc_recomb     complement(491..1096)
                     /vntifkey="86"
                     /label=ORF\603
     rep_origin      5054..5813
                     /vntifkey="33"
                     /label=pUC\Ori
     enhancer        1196..1677
                     /vntifkey="9"
                     /label=hr5
     polyA_signal    3442..3447
                     /vntifkey="25"
                     /label=PolyA\signal
     misc_feature    3410..3413
                     /vntifkey="21"
                     /label=3x\-BseR\I\TCCT\to\CTTC
     misc_feature    3179..3193
                     /vntifkey="21"
                     /label=C-Term\Ek/LIC\overhang
     misc_binding    3203..3232
                     /vntifkey="20"
                     /label=10x\His-Tag
     primer          3197..3245
                     /vntifkey="27"
                     /label=S-CTerm\10xHis
     terminator      3265..3571
                     /vntifkey="43"
                     /label=ie1\Terminator
     primer          3249..3262
                     /vntifkey="27"
                     /label=AS-Bsu36\I\Adapt\Avr
     5'UTR           2229..2278
                     /vntifkey="52"
                     /label=ie1\5'-UTR
     promoter        2279..2392
                     /vntifkey="29"
                     /label=p10\promoter\+\5'UTR
     misc_feature    2277..2280
                     /vntifkey="21"
                     /label=Bsa\I\jct
     CDS             2435..3094
                     /invitrogen="1170000"
                     /vntifkey="4"
                     /label=GST\tag\(no\stop)
     misc_feature    2395..2434
                     /vntifkey="21"
                     /label=polH\pseudo\5'-UTR\ATT
     protein_bind    3119..3133
                     /vntifkey="31"
                     /label=Ek\site
     primer          2216..2237
                     /vntifkey="27"
                     /label=IE1\Promoter\Primer
     primer          2325..2345
                     /vntifkey="27"
                     /label=p10\Promoter\primer
     primer          complement(3327..3345)
                     /vntifkey="27"
                     /label=IE1\Terminator\Primer
BASE COUNT     2030 a      1590 c      1655 g      2221 t 
ORIGIN
        1 ggaacggctc cgcccactat taatgaaatt aaaaattcca attttaaaaa acgcagcaag 
       61 agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac 
      121 aacaagatta atatgcctcc gtgtataaaa aaaatattga acgatttgaa agaaaacaat 
      181 gtaccgcgcg gcggtatgta caggaagagg tttatactaa actgttacat tgcaaacgtg 
      241 gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg ctctgacgca tttctacaac 
      301 cacgactcca agtgtgtggg tgaagtcatg catcttttaa tcaaatccca agatgtgtat 
      361 aaaccaccaa actgccaaaa aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac 
      421 tgcaagggtc tcaatcctat ttgtaattat tgaataataa aacaattata aatgctaaat 
      481 ttgtttttta ttaacgatac aaaccaaacg caacaagaac atttgtagta ttatctataa 
      541 ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa atcattttca aatgattcac 
      601 agttaatttg cgacaatata attttatttt cacataaact agacgccttg tcgtcttctt 
      661 cttcgtattc cttctctttt tcatttttct cttcataaaa attaacatag ttattatcgt 
      721 atccatatat gtatctatcg tatagagtaa attttttgtt gtcataaata tatatgtctt 
      781 ttttaatggg gtgtatagta ccgctgcgca tagtttttct gtaatttaca acagtgctat 
      841 tttctggtag ttcttcggag tgtgttgctt taattattaa atttatataa tcaatgaatt 
      901 tgggatcgtc ggttttgtac aatatgttgc cggcatagta cgcagcttct tctagttcaa 
      961 ttacaccatt ttttagcagc accggattaa cataactttc caaaatgttg tacgaaccgt 
     1021 taaacaaaaa cagttcacct cccttttcta tactattgtc tgcgagcagt tgtttgttgt 
     1081 taaaaataac agccattgta atgagacgca caaactaata tcacaaactg gaaatgtcta 
     1141 tcaatatata gttgctgatc agatctgatc atggagataa ttaaaatgat aagggcgcgt 
     1201 aaaacacaat caagtatgag tcataagctg atgtcatgtt ttgcacacgg ctcataaccg 
     1261 aactggcttt acgagtagaa ttctacttgt aacgcacgat cagtggatga tgtcatttgt 
     1321 ttttcaaatc gagatgatgt catgttttgc acacggctca taaactcgct ttacgagtag 
     1381 aattctacgt gtaacgcacg atcgattgat gagtcatttg ttttgcaata tgatatcata 
     1441 caatatgact catttgtttt tcaaaaccga acttgattta cgggtagaat tctacttgta 
     1501 aagcacaatc aaaaagatga tgtcatttgt ttttcaaaac tgaactcgct ttacgagtag 
     1561 aattctacgt gtaaaacaca atcaagaaat gatgtcattt gttataaaaa taaaagctga 
     1621 tgtcatgttt tgcacatggc tcataactaa actcgcttta cgggtagaat tctacgcgcg 
     1681 tcgatgtctt tgtgatgcgc gcgacatttt tgtaggttat tgataaaatg aacggatacg 
     1741 ttgcccgaca ttatcattaa atccttggcg tagaatttgt cgggtccatt gtccgtgtgc 
     1801 gctagcatgc ccgtaacgga cctcgtactt ttggcttcaa aggttttgcg cacagacaaa 
     1861 atgtgccaca cttgcagctc tgcatgtgtg cgcgttacca caaatcccaa cggcgcagtg 
     1921 tacttgttgt atgcaaataa atctcgataa aggcgcggcg cgcgaatgca gctgatcacg 
     1981 tacgtccctc gtgttccgtt caaggacggt gttatcgacc tcagattaat gtttatcggc 
     2041 cgactgtttt cgtatccgct caccaaacgc gtttttgcat taacattgta tgtcggcgga 
     2101 tgttctatat ctaatttgaa taaataaacg ataaccgcgt tggttttaga gggcataata 
     2161 aaagaaatat tgttatcgtg ttcgccatta gggcagtata aattgacgtt catgttggat 
     2221 attgtttcag ttgcaagttg acactggcgg cgacaagatc gtgaacaacc aagtgactcc 
     2281 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc 
     2341 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tcaatccgga 
     2401 ttattcatac cgtcccacca tcgggcgcgg atccatggcc cctatactag gttattggaa 
     2461 aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga 
     2521 agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg 
     2581 tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat 
     2641 ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg 
     2701 tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat 
     2761 tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat 
     2821 gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac 
     2881 ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg 
     2941 cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat 
     3001 tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac 
     3061 gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt ccgcgtcata atcaagcaga 
     3121 tgacgacgac aaggtaccgg atccgacgtc ggtcgacaag cttgcggccg caggagctcc 
     3181 gggcttctcc tcaatttccg ctcatcacca ccatcatcac catcaccacc actaagtgat 
     3241 taacctcacg acctaggcct caggttatac atatattttg aatttaatta attatacata 
     3301 tattttatat tatttttgtc ttttattatc gaggggccgt tgttggtgtg gggttttgca 
     3361 tagaaataac aatgggagtt ggcgacgttg ctgcgccaac accacctccc ttccctcctt 
     3421 tcatcatgta tctgtagata aaataaaata ttaaacctaa aaacaagacc gcgcctatca 
     3481 acaaaatgat aggcattaac ttgccgctga cgctgtcact aacgttggac gatttgccga 
     3541 ctaaaccttc atcgcccagt aaccaatcta ggtagctgag cgcatgcaag ctgatccggg 
     3601 ttattagtac atttattaag cgctagattc tgtgcgttgt tgatttacag acaattgttg 
     3661 tacgtatttt aataattcat taaatttata atctttaggg tggtatgtta gagcgaaaat 
     3721 caaatgattt tcagcgtctt tatatctgaa tttaaatatt aaatcctcaa tagatttgta 
     3781 aaataggttt cgattagttt caaacaaggg ttgtttttcc gaaccgatgg ctggactatc 
     3841 taatggattt tcgctcaacg ccacaaaact tgccaaatct tgtagcagca atctagcttt 
     3901 gtcgatattc gtttgtgttt tgttttgtaa taaaggttcg acgtcgttca aaatattatg 
     3961 cgcttttgta tttctttcat cactgtcgtt agtgtacaat tgactcgacg taaacacgtt 
     4021 aaatagagct tggacatatt taacatcggg cgtgttagct ttattaggcc gattatcgtc 
     4081 gtcgtcccaa ccctcgtcgt tagaagttgc ttccgaagac gattttgcca tagccacacg 
     4141 acgcctatta attgtgtcgg ctaacacgtc cgcgatcaaa tttgtagttg agctttttgg 
     4201 aattatttct gattgcgggc gtttttgggc gggtttcaat ctaactgtgc ccgattttaa 
     4261 ttcagacaac acgttagaaa gcgatggtgc aggcggtggt aacatttcag acggcaaatc 
     4321 tactaatggc ggcggtggtg gagctgatga taaatctacc atcggtggag gcgcaggcgg 
     4381 ggctggcggc ggaggcggag gcggaggtgg tggcggtgat gcagacggcg gtttaggctc 
     4441 aaatgtctct ttaggcaaca cagtcggcac ctcaactatt gtactggttt cgggcgccgt 
     4501 ttttggtttg accggtctga gacgagtgcg atttttttcg tttctaatag cttccaacaa 
     4561 ttgttgtctg tcgtctaaag gtgcagcggg ttgaggttcc gtcggcattg gtggagcggg 
     4621 cggcaattca gacatcgatg gtggtggtgg tggtggaggc gctggaatgt taggcacggg 
     4681 agaaggtggt ggcggcggtg ccgccggtat aatttgttct ggtttagttt gttcgcgcac 
     4741 gattgtgggc accggcgcag gcgccgctgg ctgcacaacg gaaggtcgtc tgcttcgagg 
     4801 cagcgcttgg ggtggtggca attcaatatt ataattggaa tacaaatcgt aaaaatctgc 
     4861 tataagcatt gtaatttcgc tatcgtttac cgtgccgata tttaacaacc gctcaatgta 
     4921 agcaattgta ttgtaaagag attgtctcaa gctcggatcg atcccgcacg ccgataacaa 
     4981 gccttttcat ttttactaca gcattgtagt ggcgagacac ttcgctgtcg tcgaggttta 
     5041 aacgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 
     5101 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 
     5161 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 
     5221 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 
     5281 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 
     5341 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 
     5401 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 
     5461 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 
     5521 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 
     5581 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 
     5641 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 
     5701 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 
     5761 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 
     5821 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 
     5881 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 
     5941 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 
     6001 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 
     6061 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 
     6121 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 
     6181 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 
     6241 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 
     6301 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 
     6361 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 
     6421 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 
     6481 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 
     6541 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 
     6601 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 
     6661 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 
     6721 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 
     6781 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 
     6841 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 
     6901 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 
     6961 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 
     7021 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 
     7081 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 
     7141 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 
     7201 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 
     7261 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 
     7321 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 
     7381 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttccca ttcgccattc 
     7441 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgcca 
//