pCTAP-Shuttle-A

创建日期:2014-07-22 查看:106
  • 出品公司 Agilent
  • 其他名称
  • 载体类型 腺病毒载体
  • 表达水平
  • 克隆方法 限制性内切酶,多克隆位点
  • 载体大小 7708 bp
  • 5'端测序 CMV Forward: CGCAAATGGGCGGTAGGCGTG
  • 3'端测序 --
  • 载体标签 SBP ; CBP (C-端)
  • 载体抗性 卡那霉素
  • 稳定性 稳表达
  • 组成
  • 病毒/非病毒 腺病毒
pCTAP Shuttle A, 7708 bp                       version 016001

NOTE: The following sequence has been verified for accuracy
at the junctions. The remainder of the sequence has been 
obtained from existing data. 


    1  CATCATCAAT AATATACCTT ATTTTGGATT GAAGCCAATA TGATAATGAG
   51  GGGGTGGAGT TTGTGACGTG GCGCGGGGCG TGGGAACGGG GCGGGTGACG
  101  TAGTAGTGTG GCGGAAGTGT GATGTTGCAA GTGTGGCGGA ACACATGTAA
  151  GCGACGGATG TGGCAAAAGT GACGTTTTTG GTGTGCGCCG GTGTACACAG
  201  GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG TAAATTTGGG
  251  CGTAACCGAG TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA
  301  AGTGAAATCT GAATAATTTT GTGTTACTCA TAGCGCGTAA TACTGTAATA
  351  GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG
  401  TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC
  451  CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG
  501  GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT
  551  TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC
  601  AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG
  651  GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA
  701  TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC
  751  TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT
  801  TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC
  851  CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
  901  AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGAGATCTG GTACCGTCGA
  951  CGCGGCCGCT CGAGCCTAAG CTTATCGATA CGCGTCCGAT CGGCAGCAAT
 1001  GGACGAGAAG ACCACCGGCT GGCGGGGCGG CCACGTGGTG GAGGGCCTGG
 1051  CCGGCGAGCT GGAGCAGCTG CGGGCCAGGC TGGAGCACCA CCCTCAGGGC
 1101  CAGCGGGAGC CCTCCGGCGG CTGCAAGCTG GGCTCCGGAA AGCGACGATG
 1151  GAAAAAGAAT TTCATAGCCG TCTCAGCAGC CAACCGCTTT AAGAAAATCT
 1201  CATCCTCCGG GGCACTTTAG GATATCCGAT CCACCGGATC TAGATAACTG
 1251  ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA
 1301  CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG
 1351  TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC
 1401  ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG
 1451  TTTGTCCAAA CTCATCAATG TATCTTAACG CGGATCTGGG CGTGGTTAAG
 1501  GGTGGGAAAG AATATATAAG GTGGGGGTCT TATGTAGTTT TGTATCTGTT
 1551  TTGCAGCAGC CGCCGCCGCC ATGAGCACCA ACTCGTTTGA TGGAAGCATT
 1601  GTGAGCTCAT ATTTGACAAC GCGCATGCCC CCATGGGCCG GGGTGCGTCA
 1651  GAATGTGATG GGCTCCAGCA TTGATGGTCG CCCCGTCCTG CCCGCAAACT
 1701  CTACTACCTT GACCTACGAG ACCGTGTCTG GAACGCCGTT GGAGACTGCA
 1751  GCCTCCGCCG CCGCTTCAGC CGCTGCAGCC ACCGCCCGCG GGATTGTGAC
 1801  TGACTTTGCT TTCCTGAGCC CGCTTGCAAG CAGTGCAGCT TCCCGTTCAT
 1851  CCGCCCGCGA TGACAAGTTG ACGGCTCTTT TGGCACAATT GGATTCTTTG
 1901  ACCCGGGAAC TTAATGTCGT TTCTCAGCAG CTGTTGGATC TGCGCCAGCA
 1951  GGTTTCTGCC CTGAAGGCTT CCTCCCCTCC CAATGCGGTT TAAAACATAA
 2001  ATAAAAAACC AGACTCTGTT TGGATTTGGA TCAAGCAAGT GTCTTGCTGT
 2051  CTTTATTTAG GGGTTTTGCG CGCGCGGTAG GCCCGGGACC AGCGGTCTCG
 2101  GTCGTTGAGG GTCCTGTGTA TTTTTTCCAG GACGTGGTAA AGGTGACTCT
 2151  GGATGTTCAG ATACATGGGC ATAAGCCCGT CTCTGGGGTG GAGGTAGCAC
 2201  CACTGCAGAG CTTCATGCTG CGGGGTGGTG TTGTAGATGA TCCAGTCGTA
 2251  GCAGGAGCGC TGGGCGTGGT GCCTAAAAAT GTCTTTCAGT AGCAAGCTGA
 2301  TTGCCAGGGG CAGGCCCTTG GTGTAAGTGT TTACAAAGCG GTTAAGCTGG
 2351  GATGGGTGCA TACGTGGGGA TATGAGATGC ATCTTGGACT GTATTTTTAG
 2401  GTTGGCTATG TTCCCAGCCA TATCCCTCCG GGGATTCATG TTGTGCAGAA
 2451  CCACCAGCAC AGTGTATCCG GTGCACTTGG GAAATTTGTC ATGTAGCTTA
 2501  GAAGGAAATG CGTGGAAGAA CTTGGAGACG CCCTTGTGAC CTCCAAGATT
 2551  TTCCATGCAT TCGTCCATAA TGATGGCAAT GGGCCCACGG GCGGCGGCCT
 2601  GGGCGAAGAT ATTTCTGGGA TCACTAACGT CATAGTTGTG TTCCAGGATG
 2651  AGATCGTCAT AGGCCATTTT TACAAAGCGC GGGCGGAGGG TGCCAGACTG
 2701  CGGTATAATG GTTCCATCCG GCCCAGGGGC GTAGTTACCC TCACAGATTT
 2751  GCATTTCCCA CGCTTTGAGT TCAGATGGGG GGATCATGTC TACCTGCGGG
 2801  GCGATGAAGA AAACGGTTTC CGGGGTAGGG GAGATCAGCT GGGAAGAAAG
 2851  CAGGTTCCTG AGCAGCTGCG ACTTACCGCA GCCGGTGGGC CCGTAAATCA
 2901  CACCTATTAC CGGCTGCAAC TGGTAGTTAA GAGAGCTGCA GCTGCCGTCA
 2951  TCCCTGAGCA GGGGGGCCAC TTCGTTAAGC ATGTCCCTGA CTCGCATGTT
 3001  TTCCCTGACC AAATCCGCCA GAAGGCGCTC GCCGCCCAGC GATAGCAGTT
 3051  CTTGCAAGGA AGCAAAGTTT TTCAACGGTT TGAGACCGTC CGCCGTAGGC
 3101  ATGCTTTTGA GCGTTTGACC AAGCAGTTCC AGGCGGTCCC ACAGCTCGGT
 3151  CACCTGCTCT ACGGCATCTC GATCCAGCAT ATCTCCTCGT TTCGCGGGTT
 3201  GGGGCGGCTT TCGCTGTACG GCAGTAGTCG GTGCTCGTCC AGACGGGCCA
 3251  GGGTCATGTC TTTCCACGGG CGCAGGGTCC TCGTCAGCGT AGTCTGGGTC
 3301  ACGGTGAAGG GGTGCGCTCC GGGCTGCGCG CTGGCCAGGG TGCGCTTGAG
 3351  GCTGGTCCTG CTGGTGCTGA AGCGCTGCCG GTCTTCGCCC TGCGCGTCGG
 3401  CCAGGTAGCA TTTGACCATG GTGTCATAGT CCAGCCCCTC CGCGGCGTGG
 3451  CCCTTGGCGC GCAGCTTGCC CTTGGAGGAG GCGCCGCACG AGGGGCAGTG
 3501  CAGACTTTTG AGGGCGTAGA GCTTGGGCGC GAGAAATACC GATTCCGGGG
 3551  AGTAGGCATC CGCGCCGCAG GCCCCGCAGA CGGTCTCGCA TTCCACGAGC
 3601  CAGGTGAGCT CTGGCCGTTC GGGGTCAAAA ACCAGGTTTC CCCCATGCTT
 3651  TTTGATGCGT TTCTTACCTC TGGTTTCCAT GAGCCGGTGT CCACGCTCGG
 3701  TGACGAAAAG GCTGTCCGTG TCCCCGTATA CAGACTTGAG AGGGAGTTTA
 3751  AACGAATTCA ATAGCTTGTT GCATGGGCGG CGATATAAAA TGCAAGGTGC
 3801  TGCTCAAAAA ATCAGGCAAA GCCTCGCGCA AAAAAGAAAG CACATCGTAG
 3851  TCATGCTCAT GCAGATAAAG GCAGGTAAGC TCCGGAACCA CCACAGAAAA
 3901  AGACACCATT TTTCTCTCAA ACATGTCTGC GGGTTTCTGC ATAAACACAA
 3951  AATAAAATAA CAAAAAAACA TTTAAACATT AGAAGCCTGT CTTACAACAG
 4001  GAAAAACAAC CCTTATAAGC ATAAGACGGA CTACGGCCAT GCCGGCGTGA
 4051  CCGTAAAAAA ACTGGTCACC GTGATTAAAA AGCACCACCG ACAGCTCCTC
 4101  GGTCATGTCC GGAGTCATAA TGTAAGACTC GGTAAACACA TCAGGTTGAT
 4151  TCACATCGGT CAGTGCTAAA AAGCGACCGA AATAGCCCGG GGGAATACAT
 4201  ACCCGCAGGC GTAGAGACAA CATTACAGCC CCCATAGGAG GTATAACAAA
 4251  ATTAATAGGA GAGAAAAACA CATAAACACC TGAAAAACCC TCCTGCCTAG
 4301  GCAAAATAGC ACCCTCCCGC TCCAGAACAA CATACAGCGC TTCCACAGCG
 4351  GCAGCCATAA CAGTCAGCCT TACCAGTAAA AAAGAAAACC TATTAAAAAA
 4401  ACACCACTCG ACACGGCACC AGCTCAATCA GTCACAGTGT AAAAAAGGGC
 4451  CAAGTGCAGA GCGAGTATAT ATAGGACTAA AAAATGACGT AACGGTTAAA
 4501  GTCCACAAAA AACACCCAGA AAACCGCACG CGAACCTACG CCCAGAAACG
 4551  AAAGCCAAAA AACCCACAAC TTCCTCAAAT CGTCACTTCC GTTTTCCCAC
 4601  GTTACGTCAC TTCCCATTTT AAGAAAACTA CAATTCCCAA CACATACAAG
 4651  TTACTCCGCC CTAAAACCTA CGTCACCCGC CCCGTTCCCA CGCCCCGCGC
 4701  CACGTCACAA ACTCCACCCC CTCATTATCA TATTGGCTTC AATCCAAAAT
 4751  AAGGTATATT ATTGATGATG TTAATTAACA TGCATGGATC CATATGCGGT
 4801  GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCTCT
 4851  TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG
 4901  AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG
 4951  GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG
 5001  AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC
 5051  TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA
 5101  CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC
 5151  TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
 5201  TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT
 5251  CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT
 5301  CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
 5351  GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA
 5401  GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT
 5451  AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA
 5501  GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
 5551  CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC
 5601  AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA
 5651  CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT
 5701  CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
 5751  TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT
 5801  AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
 5851  TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
 5901  TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC
 5951  AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG
 6001  GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
 6051  GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT
 6101  TGCTGCAGCC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTCA
 6151  CGTAGAAAGC CAGTCCGCAG AAACGGTGCT GACCCCGGAT GAATGTCAGC
 6201  TACTGGGCTA TCTGGACAAG GGAAAACGCA AGCGCAAAGA GAAAGCAGGT
 6251  AGCTTGCAGT GGGCTTACAT GGCGATAGCT AGACTGGGCG GTTTTATGGA
 6301  CAGCAAGCGA ACCGGAATTG CCAGCTGGGG CGCCCTCTGG TAAGGTTGGG
 6351  AAGCCCTGCA AAGTAAACTG GATGGCTTTC TTGCCGCCAA GGATCTGATG
 6401  GCGCAGGGGA TCAAGCTCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA
 6451  TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
 6501  AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC
 6551  CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA
 6601  CCGACCTGTC CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA
 6651  TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT
 6701  CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG
 6751  ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
 6801  GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA
 6851  CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG
 6901  GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA
 6951  GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG GCGAGGATCT
 7001  CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG
 7051  GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
 7101  TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG
 7151  CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT
 7201  CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAATTTTG
 7251  TTAAAATTTT TGTTAAATCA GCTCATTTTT TAACCAATAG GCCGAAATCG
 7301  GCACCATCCC TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT
 7351  GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG ACTCCAACGT
 7401  CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT
 7451  CACCCTAATC AAGTTTTTTG TGGTCGAGGT GCCGTAAAGC ACTAAATCGG
 7501  AACCCTAAAG GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA
 7551  CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC
 7601  TGGCAAGTGT AGCGGTCACG CTGCGCGTAA CCACCACACC CGCGCGCTTA
 7651  ATGCGCCGCT ACAGGGCGCG TCCATTCGCC ATTCAGGATC GAATTAATTC
 7701  TTAATTAA
科研狗©2015-2024 科研好助手,京ICP备20005780号-1 建议意见

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