pCTAP-Shuttle-B

创建日期:2013-02-22 查看:166
  • 出品公司 Agilent
  • 其他名称
  • 载体类型 腺病毒载体
  • 表达水平
  • 克隆方法 限制性内切酶,多克隆位点
  • 载体大小 7709 bp
  • 5'端测序 CMV Forward: CGCAAATGGGCGGTAGGCGTG
  • 3'端测序 --
  • 载体标签 SBP; CBP(C-端)
  • 载体抗性 卡那霉素
  • 稳定性 稳表达
  • 组成
  • 病毒/非病毒 腺病毒
pCTAP Shuttle B, 7709 bp                       version 016001

NOTE: The following sequence has been verified for accuracy
at the junctions. The remainder of the sequence has been 
obtained from existing data. 


    1  CATCATCAAT AATATACCTT ATTTTGGATT GAAGCCAATA TGATAATGAG
   51  GGGGTGGAGT TTGTGACGTG GCGCGGGGCG TGGGAACGGG GCGGGTGACG
  101  TAGTAGTGTG GCGGAAGTGT GATGTTGCAA GTGTGGCGGA ACACATGTAA
  151  GCGACGGATG TGGCAAAAGT GACGTTTTTG GTGTGCGCCG GTGTACACAG
  201  GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG TAAATTTGGG
  251  CGTAACCGAG TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA
  301  AGTGAAATCT GAATAATTTT GTGTTACTCA TAGCGCGTAA TACTGTAATA
  351  GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG
  401  TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC
  451  CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG
  501  GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT
  551  TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC
  601  AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG
  651  GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA
  701  TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC
  751  TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT
  801  TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC
  851  CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
  901  AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGAGATCTG GTACCGTCGA
  951  CGCGGCCGCT CGAGCCTAAG CTTATCGATA CGCGTCCGAT CGAGCAGCAA
 1001  TGGACGAGAA GACCACCGGC TGGCGGGGCG GCCACGTGGT GGAGGGCCTG
 1051  GCCGGCGAGC TGGAGCAGCT GCGGGCCAGG CTGGAGCACC ACCCTCAGGG
 1101  CCAGCGGGAG CCCTCCGGCG GCTGCAAGCT GGGCTCCGGA AAGCGACGAT
 1151  GGAAAAAGAA TTTCATAGCC GTCTCAGCAG CCAACCGCTT TAAGAAAATC
 1201  TCATCCTCCG GGGCACTTTA GGATATCCGA TCCACCGGAT CTAGATAACT
 1251  GATCATAATC AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA
 1301  ACCTCCCACA CCTCCCCCTG AACCTGAAAC ATAAAATGAA TGCAATTGTT
 1351  GTTGTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG
 1401  CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG
 1451  GTTTGTCCAA ACTCATCAAT GTATCTTAAC GCGGATCTGG GCGTGGTTAA
 1501  GGGTGGGAAA GAATATATAA GGTGGGGGTC TTATGTAGTT TTGTATCTGT
 1551  TTTGCAGCAG CCGCCGCCGC CATGAGCACC AACTCGTTTG ATGGAAGCAT
 1601  TGTGAGCTCA TATTTGACAA CGCGCATGCC CCCATGGGCC GGGGTGCGTC
 1651  AGAATGTGAT GGGCTCCAGC ATTGATGGTC GCCCCGTCCT GCCCGCAAAC
 1701  TCTACTACCT TGACCTACGA GACCGTGTCT GGAACGCCGT TGGAGACTGC
 1751  AGCCTCCGCC GCCGCTTCAG CCGCTGCAGC CACCGCCCGC GGGATTGTGA
 1801  CTGACTTTGC TTTCCTGAGC CCGCTTGCAA GCAGTGCAGC TTCCCGTTCA
 1851  TCCGCCCGCG ATGACAAGTT GACGGCTCTT TTGGCACAAT TGGATTCTTT
 1901  GACCCGGGAA CTTAATGTCG TTTCTCAGCA GCTGTTGGAT CTGCGCCAGC
 1951  AGGTTTCTGC CCTGAAGGCT TCCTCCCCTC CCAATGCGGT TTAAAACATA
 2001  AATAAAAAAC CAGACTCTGT TTGGATTTGG ATCAAGCAAG TGTCTTGCTG
 2051  TCTTTATTTA GGGGTTTTGC GCGCGCGGTA GGCCCGGGAC CAGCGGTCTC
 2101  GGTCGTTGAG GGTCCTGTGT ATTTTTTCCA GGACGTGGTA AAGGTGACTC
 2151  TGGATGTTCA GATACATGGG CATAAGCCCG TCTCTGGGGT GGAGGTAGCA
 2201  CCACTGCAGA GCTTCATGCT GCGGGGTGGT GTTGTAGATG ATCCAGTCGT
 2251  AGCAGGAGCG CTGGGCGTGG TGCCTAAAAA TGTCTTTCAG TAGCAAGCTG
 2301  ATTGCCAGGG GCAGGCCCTT GGTGTAAGTG TTTACAAAGC GGTTAAGCTG
 2351  GGATGGGTGC ATACGTGGGG ATATGAGATG CATCTTGGAC TGTATTTTTA
 2401  GGTTGGCTAT GTTCCCAGCC ATATCCCTCC GGGGATTCAT GTTGTGCAGA
 2451  ACCACCAGCA CAGTGTATCC GGTGCACTTG GGAAATTTGT CATGTAGCTT
 2501  AGAAGGAAAT GCGTGGAAGA ACTTGGAGAC GCCCTTGTGA CCTCCAAGAT
 2551  TTTCCATGCA TTCGTCCATA ATGATGGCAA TGGGCCCACG GGCGGCGGCC
 2601  TGGGCGAAGA TATTTCTGGG ATCACTAACG TCATAGTTGT GTTCCAGGAT
 2651  GAGATCGTCA TAGGCCATTT TTACAAAGCG CGGGCGGAGG GTGCCAGACT
 2701  GCGGTATAAT GGTTCCATCC GGCCCAGGGG CGTAGTTACC CTCACAGATT
 2751  TGCATTTCCC ACGCTTTGAG TTCAGATGGG GGGATCATGT CTACCTGCGG
 2801  GGCGATGAAG AAAACGGTTT CCGGGGTAGG GGAGATCAGC TGGGAAGAAA
 2851  GCAGGTTCCT GAGCAGCTGC GACTTACCGC AGCCGGTGGG CCCGTAAATC
 2901  ACACCTATTA CCGGGTGCAA CTGGTAGTTA AGAGAGCTGC AGCTGCCGTC
 2951  ATCCCTGAGC AGGGGGGCCA CTTCGTTAAG CATGTCCCTG ACTCGCATGT
 3001  TTTCCCTGAC CAAATCCGCC AGAAGGCGCT CGCCGCCCAG CGATAGCAGT
 3051  TCTTGCAAGG AAGCAAAGTT TTTCAACGGT TTGAGACCGT CCGCCGTAGG
 3101  CATGCTTTTG AGCGTTTGAC CAAGCAGTTC CAGGCGGTCC CACAGCTCGG
 3151  TCACCTGCTC TACGGCATCT CGATCCAGCA TATCTCCTCG TTTCGCGGGT
 3201  TGGGGCGGCT TTCGCTGTAC GGCAGTAGTC GGTGCTCGTC CAGACGGGCC
 3251  AGGGTCATGT CTTTCCACGG GCGCAGGGTC CTCGTCAGCG TAGTCTGGGT
 3301  CACGGTGAAG GGGTGCGCTC CGGGCTGCGC GCTGGCCAGG GTGCGCTTGA
 3351  GGCTGGTCCT GCTGGTGCTG AAGCGCTGCC GGTCTTCGCC CTGCGCGTCG
 3401  GCCAGGTAGC ATTTGACCAT GGTGTCATAG TCCAGCCCCT CCGCGGCGTG
 3451  GCCCTTGGCG CGCAGCTTGC CCTTGGAGGA GGCGCCGCAC GAGGGGCAGT
 3501  GCAGACTTTT GAGGGCGTAG AGCTTGGGCG CGAGAAATAC CGATTCCGGG
 3551  GAGTAGGCAT CCGCGCCGCA GGCCCCGCAG ACGGTCTCGC ATTCCACGAG
 3601  CCAGGTGAGC TCTGGCCGTT CGGGGTCAAA AACCAGGTTT CCCCCATGCT
 3651  TTTTGATGCG TTTCTTACCT CTGGTTTCCA TGAGCCGGTG TCCACGCTCG
 3701  GTGACGAAAA GGCTGTCCGT GTCCCCGTAT ACAGACTTGA GAGGGAGTTT
 3751  AAACGAATTC AATAGCTTGT TGCATGGGCG GCGATATAAA ATGCAAGGTG
 3801  CTGCTCAAAA AATCAGGCAA AGCCTCGCGC AAAAAAGAAA GCACATCGTA
 3851  GTCATGCTCA TGCAGATAAA GGCAGGTAAG CTCCGGAACC ACCACAGAAA
 3901  AAGACACCAT TTTTCTCTCA AACATGTCTG CGGGTTTCTG CATAAACACA
 3951  AAATAAAATA ACAAAAAAAC ATTTAAACAT TAGAAGCCTG TCTTACAACA
 4001  GGAAAAACAA CCCTTATAAG CATAAGACGG ACTACGGCCA TGCCGGCGTG
 4051  ACCGTAAAAA AACTGGTCAC CGTGATTAAA AAGCACCACC GACAGCTCCT
 4101  CGGTCATGTC CGGAGTCATA ATGTAAGACT CGGTAAACAC ATCAGGTTGA
 4151  TTCACATCGG TCAGTGCTAA AAAGCGACCG AAATAGCCCG GGGGAATACA
 4201  TACCCGCAGG CGTAGAGACA ACATTACAGC CCCCATAGGA GGTATAACAA
 4251  AATTAATAGG AGAGAAAAAC ACATAAACAC CTGAAAAACC CTCCTGCCTA
 4301  GGCAAAATAG CACCCTCCCG CTCCAGAACA ACATACAGCG CTTCCACAGC
 4351  GGCAGCCATA ACAGTCAGCC TTACCAGTAA AAAAGAAAAC CTATTAAAAA
 4401  AACACCACTC GACACGGCAC CAGCTCAATC AGTCACAGTG TAAAAAAGGG
 4451  CCAAGTGCAG AGCGAGTATA TATAGGACTA AAAAATGACG TAACGGTTAA
 4501  AGTCCACAAA AAACACCCAG AAAACCGCAC GCGAACCTAC GCCCAGAAAC
 4551  GAAAGCCAAA AAACCCACAA CTTCCTCAAA TCGTCACTTC CGTTTTCCCA
 4601  CGTTACGTCA CTTCCCATTT TAAGAAAACT ACAATTCCCA ACACATACAA
 4651  GTTACTCCGC CCTAAAACCT ACGTCACCCG CCCCGTTCCC ACGCCCCGCG
 4701  CCACGTCACA AACTCCACCC CCTCATTATC ATATTGGCTT CAATCCAAAA
 4751  TAAGGTATAT TATTGATGAT GTTAATTAAC ATGCATGGAT CCATATGCGG
 4801  TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCTC
 4851  TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC
 4901  GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA
 4951  GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG
 5001  GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC
 5051  CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG
 5101  ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
 5151  CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC
 5201  CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT
 5251  TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT
 5301  TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC
 5351  CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT
 5401  AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
 5451  TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA
 5501  AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA
 5551  ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG
 5601  CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG
 5651  ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA
 5701  TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
 5751  ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT
 5801  TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
 5851  GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC
 5901  ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC
 5951  CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT
 6001  GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
 6051  AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA
 6101  TTGCTGCAGC CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTC
 6151  ACGTAGAAAG CCAGTCCGCA GAAACGGTGC TGACCCCGGA TGAATGTCAG
 6201  CTACTGGGCT ATCTGGACAA GGGAAAACGC AAGCGCAAAG AGAAAGCAGG
 6251  TAGCTTGCAG TGGGCTTACA TGGCGATAGC TAGACTGGGC GGTTTTATGG
 6301  ACAGCAAGCG AACCGGAATT GCCAGCTGGG GCGCCCTCTG GTAAGGTTGG
 6351  GAAGCCCTGC AAAGTAAACT GGATGGCTTT CTTGCCGCCA AGGATCTGAT
 6401  GGCGCAGGGG ATCAAGCTCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC
 6451  ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
 6501  GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG
 6551  CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG
 6601  ACCGACCTGT CCGGTGCCCT GAATGAACTG CAAGACGAGG CAGCGCGGCT
 6651  ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG
 6701  TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG
 6751  GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
 6801  TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG
 6851  ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC
 6901  GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC
 6951  AGCCGAACTG TTCGCCAGGC TCAAGGCGAG CATGCCCGAC GGCGAGGATC
 7001  TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT
 7051  GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
 7101  CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG
 7151  GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT
 7201  TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAATTTT
 7251  GTTAAAATTT TTGTTAAATC AGCTCATTTT TTAACCAATA GGCCGAAATC
 7301  GGCACCATCC CTTATAAATC AAAAGAATAG ACCGAGATAG GGTTGAGTGT
 7351  TGTTCCAGTT TGGAACAAGA GTCCACTATT AAAGAACGTG GACTCCAACG
 7401  TCAAAGGGCG AAAAACCGTC TATCAGGGCG ATGGCCCACT ACGTGAACCA
 7451  TCACCCTAAT CAAGTTTTTT GTGGTCGAGG TGCCGTAAAG CACTAAATCG
 7501  GAACCCTAAA GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA
 7551  ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG CGCTAGGGCG
 7601  CTGGCAAGTG TAGCGGTCAC GCTGCGCGTA ACCACCACAC CCGCGCGCTT
 7651  AATGCGCCGC TACAGGGCGC GTCCATTCGC CATTCAGGAT CGAATTAATT
 7701  CTTAATTAA
科研狗©2015-2024 科研好助手,京ICP备20005780号-1 建议意见

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