pCTAP-Shuttle-C

创建日期:2013-02-18 查看:125
  • 出品公司 Agilent
  • 其他名称
  • 载体类型 腺病毒载体
  • 表达水平
  • 克隆方法 限制性内切酶,多克隆位点
  • 载体大小 7710 bp
  • 5'端测序 CMV Forward: CGCAAATGGGCGGTAGGCGTG
  • 3'端测序 --
  • 载体标签 SBP; CBP (C-端)
  • 载体抗性 卡那霉素
  • 稳定性 稳表达
  • 组成
  • 病毒/非病毒 腺病毒
pCTAP Shuttle C, 7710 bp                       version 016001

NOTE: The following sequence has been verified for accuracy
at the junctions. The remainder of the sequence has been 
obtained from existing data. 


    1  CATCATCAAT AATATACCTT ATTTTGGATT GAAGCCAATA TGATAATGAG
   51  GGGGTGGAGT TTGTGACGTG GCGCGGGGCG TGGGAACGGG GCGGGTGACG
  101  TAGTAGTGTG GCGGAAGTGT GATGTTGCAA GTGTGGCGGA ACACATGTAA
  151  GCGACGGATG TGGCAAAAGT GACGTTTTTG GTGTGCGCCG GTGTACACAG
  201  GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG TAAATTTGGG
  251  CGTAACCGAG TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA
  301  AGTGAAATCT GAATAATTTT GTGTTACTCA TAGCGCGTAA TACTGTAATA
  351  GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG
  401  TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC
  451  CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG
  501  GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT
  551  TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC
  601  AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG
  651  GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA
  701  TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC
  751  TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT
  801  TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC
  851  CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
  901  AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGAGATCTG GTACCGTCGA
  951  CGCGGCCGCT CGAGCCTAAG CTTATCGATA CGCGTCCGAT CGGAGCAGCA
 1001  ATGGACGAGA AGACCACCGG CTGGCGGGGC GGCCACGTGG TGGAGGGCCT
 1051  GGCCGGCGAG CTGGAGCAGC TGCGGGCCAG GCTGGAGCAC CACCCTCAGG
 1101  GCCAGCGGGA GCCCTCCGGC GGCTGCAAGC TGGGCTCCGG AAAGCGACGA
 1151  TGGAAAAAGA ATTTCATAGC CGTCTCAGCA GCCAACCGCT TTAAGAAAAT
 1201  CTCATCCTCC GGGGCACTTT AGGATATCCG ATCCACCGGA TCTAGATAAC
 1251  TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA
 1301  AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT
 1351  TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA
 1401  GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT
 1451  GGTTTGTCCA AACTCATCAA TGTATCTTAA CGCGGATCTG GGCGTGGTTA
 1501  AGGGTGGGAA AGAATATATA AGGTGGGGGT CTTATGTAGT TTTGTATCTG
 1551  TTTTGCAGCA GCCGCCGCCG CCATGAGCAC CAACTCGTTT GATGGAAGCA
 1601  TTGTGAGCTC ATATTTGACA ACGCGCATGC CCCCATGGGC CGGGGTGCGT
 1651  CAGAATGTGA TGGGCTCCAG CATTGATGGT CGCCCCGTCC TGCCCGCAAA
 1701  CTCTACTACC TTGACCTACG AGACCGTGTC TGGAACGCCG TTGGAGACTG
 1751  CAGCCTCCGC CGCCGCTTCA GCCGCTGCAG CCACCGCCCG CGGGATTGTG
 1801  ACTGACTTTG CTTTCCTGAG CCCGCTTGCA AGCAGTGCAG CTTCCCGTTC
 1851  ATCCGCCCGC GATGACAAGT TGACGGCTCT TTTGGCACAA TTGGATTCTT
 1901  TGACCCGGGA ACTTAATGTC GTTTCTCAGC AGCTGTTGGA TCTGCGCCAG
 1951  CAGGTTTCTG CCCTGAAGGC TTCCTCCCCT CCCAATGCGG TTTAAAACAT
 2001  AAATAAAAAA CCAGACTCTG TTTGGATTTG GATCAAGCAA GTGTCTTGCT
 2051  GTCTTTATTT AGGGGTTTTG CGCGCGCGGT AGGCCCGGGA CCAGCGGTCT
 2101  CGGTCGTTGA GGGTCCTGTG TATTTTTTCC AGGACGTGGT AAAGGTGACT
 2151  CTGGATGTTC AGATACATGG GCATAAGCCC GTCTCTGGGG TGGAGGTAGC
 2201  ACCACTGCAG AGCTTCATGC TGCGGGGTGG TGTTGTAGAT GATCCAGTCG
 2251  TAGCAGGAGC GCTGGGCGTG GTGCCTAAAA ATGTCTTTCA GTAGCAAGCT
 2301  GATTGCCAGG GGCAGGCCCT TGGTGTAAGT GTTTACAAAG CGGTTAAGCT
 2351  GGGATGGGTG CATACGTGGG GATATGAGAT GCATCTTGGA CTGTATTTTT
 2401  AGGTTGGCTA TGTTCCCAGC CATATCCCTC CGGGGATTCA TGTTGTGCAG
 2451  AACCACCAGC ACAGTGTATC CGGTGCACTT GGGAAATTTG TCATGTAGCT
 2501  TAGAAGGAAA TGCGTGGAAG AACTTGGAGA CGCCCTTGTG ACCTCCAAGA
 2551  TTTTCCATGC ATTCGTCCAT AATGATGGCA ATGGGCCCAC GGGCGGCGGC
 2601  CTGGGCGAAG ATATTTCTGG GATCACTAAC GTCATAGTTG TGTTCCAGGA
 2651  TGAGATCGTC ATAGGCCATT TTTACAAAGC GCGGGCGGAG GGTGCCAGAC
 2701  TGCGGTATAA TGGTTCCATC CGGCCCAGGG GCGTAGTTAC CCTCACAGAT
 2751  TTGCATTTCC CACGCTTTGA GTTCAGATGG GGGGATCATG TCTACCTGCG
 2801  GGGCGATGAA GAAAACGGTT TCCGGGGTAG GGGAGATCAG CTGGGAAGAA
 2851  AGCAGGTTCC TGAGCAGCTG CGACTTACCG CAGCCGGTGG GCCCGTAAAT
 2901  CACACCTATT ACCGGGTGCA ACTGGTAGTT AAGAGAGCTG CAGCTGCCGT
 2951  CATCCCTGAG CAGGGGGGCC ACTTCGTTAA GCATGTCCCT GACTCGCATG
 3001  TTTTCCCTGA CCAAATCCGC CAGAAGGCGC TCGCCGCCCA GCGATAGCAG
 3051  TTCTTGCAAG GAAGCAAAGT TTTTCAACGG TTTGAGACCG TCCGCCGTAG
 3101  GCATGCTTTT GAGCGTTTGA CCAAGCAGTT CCAGGCGGTC CCACAGCTCG
 3151  GTCACCTGCT CTACGGCATC TCGATCCAGC ATATCTCCTC GTTTCGCGGG
 3201  TTGGGGCGGC TTTCGCTGTA CGGCAGTAGT CGGTGCTCGT CCAGACGGGC
 3251  CAGGGTCATG TCTTTCCACG GGCGCAGGGT CCTCGTCAGC GTAGTCTGGG
 3301  TCACGGTGAA GGGGTGCGCT CCGGGCTGCG CGCTGGCCAG GGTGCGCTTG
 3351  AGGCTGGTCC TGCTGGTGCT GAAGCGCTGC CGGTCTTCGC CCTGCGCGTC
 3401  GGCCAGGTAG CATTTGACCA TGGTGTCATA GTCCAGCCCC TCCGCGGCGT
 3451  GGCCCTTGGC GCGCAGCTTG CCCTTGGAGG AGGCGCCGCA CGAGGGGCAG
 3501  TGCAGACTTT TGAGGGCGTA GAGCTTGGGC GCGAGAAATA CCGATTCCGG
 3551  GGAGTAGGCA TCCGCGCCGC AGGCCCCGCA GACGGTCTCG CATTCCACGA
 3601  GCCAGGTGAG CTCTGGCCGT TCGGGGTCAA AAACCAGGTT TCCCCCATGC
 3651  TTTTTGATGC GTTTCTTACC TCTGGTTTCC ATGAGCCGGT GTCCACGCTC
 3701  GGTGACGAAA AGGCTGTCCG TGTCCCCGTA TACAGACTTG AGAGGGAGTT
 3751  TAAACGAATT CAATAGCTTG TTGCATGGGC GGCGATATAA AATGCAAGGT
 3801  GCTGCTCAAA AAATCAGGCA AAGCCTCGCG CAAAAAAGAA AGCACATCGT
 3851  AGTCATGCTC ATGCAGATAA AGGCAGGTAA GCTCCGGAAC CACCACAGAA
 3901  AAAGACACCA TTTTTCTCTC AAACATGTCT GCGGGTTTCT GCATAAACAC
 3951  AAAATAAAAT AACAAAAAAA CATTTAAACA TTAGAAGCCT GTCTTACAAC
 4001  AGGAAAAACA ACCCTTATAA GCATAAGACG GACTACGGCC ATGCCGGCGT
 4051  GACCGTAAAA AAACTGGTCA CCGTGATTAA AAAGCACCAC CGACAGCTCC
 4101  TCGGTCATGT CCGGAGTCAT AATGTAAGAC TCGGTAAACA CATCAGGTTG
 4151  ATTCACATCG GTCAGTGCTA AAAAGCGACC GAAATAGCCC GGGGGAATAC
 4201  ATACCCGCAG GCGTAGAGAC AACATTACAG CCCCCATAGG AGGTATAACA
 4251  AAATTAATAG GAGAGAAAAA CACATAAACA CCTGAAAAAC CCTCCTGCCT
 4301  AGGCAAAATA GCACCCTCCC GCTCCAGAAC AACATACAGC GCTTCCACAG
 4351  CGGCAGCCAT AACAGTCAGC CTTACCAGTA AAAAAGAAAA CCTATTAAAA
 4401  AAACACCACT CGACACGGCA CCAGCTCAAT CAGTCACAGT GTAAAAAAGG
 4451  GCCAAGTGCA GAGCGAGTAT ATATAGGACT AAAAAATGAC GTAACGGTTA
 4501  AAGTCCACAA AAAACACCCA GAAAACCGCA CGCGAACCTA CGCCCAGAAA
 4551  CGAAAGCCAA AAAACCCACA ACTTCCTCAA ATCGTCACTT CCGTTTTCCC
 4601  ACGTTACGTC ACTTCCCATT TTAAGAAAAC TACAATTCCC AACACATACA
 4651  AGTTACTCCG CCCTAAAACC TACGTCACCC GCCCCGTTCC CACGCCCCGC
 4701  GCCACGTCAC AAACTCCACC CCCTCATTAT CATATTGGCT TCAATCCAAA
 4751  ATAAGGTATA TTATTGATGA TGTTAATTAA CATGCATGGA TCCATATGCG
 4801  GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT
 4851  CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG
 4901  CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC
 4951  AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA
 5001  GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC
 5051  CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC
 5101  GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC
 5151  GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC
 5201  CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG
 5251  TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG
 5301  TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
 5351  CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT
 5401  TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC
 5451  CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG
 5501  AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA
 5551  AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC
 5601  GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT
 5651  GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT
 5701  ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA
 5751  AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
 5801  TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT
 5851  AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC
 5901  CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT
 5951  CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG
 6001  TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG
 6051  AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
 6101  ATTGCTGCAG CCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT
 6151  CACGTAGAAA GCCAGTCCGC AGAAACGGTG CTGACCCCGG ATGAATGTCA
 6201  GCTACTGGGC TATCTGGACA AGGGAAAACG CAAGCGCAAA GAGAAAGCAG
 6251  GTAGCTTGCA GTGGGCTTAC ATGGCGATAG CTAGACTGGG CGGTTTTATG
 6301  GACAGCAAGC GAACCGGAAT TGCCAGCTGG GGCGCCCTCT GGTAAGGTTG
 6351  GGAAGCCCTG CAAAGTAAAC TGGATGGCTT TCTTGCCGCC AAGGATCTGA
 6401  TGGCGCAGGG GATCAAGCTC TGATCAAGAG ACAGGATGAG GATCGTTTCG
 6451  CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG
 6501  AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT
 6551  GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
 6601  GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG GCAGCGCGGC
 6651  TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT
 6701  GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA
 6751  GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG
 6801  CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC
 6851  GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC
 6901  CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC
 6951  CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA CGGCGAGGAT
 7001  CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA
 7051  TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC
 7101  GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC
 7151  GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA
 7201  TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAATTT
 7251  TGTTAAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT
 7301  CGGCACCATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG
 7351  TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC
 7401  GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC
 7451  ATCACCCTAA TCAAGTTTTT TGTGGTCGAG GTGCCGTAAA GCACTAAATC
 7501  GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG
 7551  AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC
 7601  GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCGCGCT
 7651  TAATGCGCCG CTACAGGGCG CGTCCATTCG CCATTCAGGA TCGAATTAAT
 7701  TCTTAATTAA
科研狗©2015-2024 科研好助手,京ICP备20005780号-1 建议意见

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