pNTAP-Shuttle-A

创建日期:2015-07-21 查看:110
  • 出品公司 Agilent
  • 其他名称
  • 载体类型 腺病毒载体
  • 表达水平
  • 克隆方法 限制性内切酶,多克隆位点
  • 载体大小 7735 bp
  • 5'端测序 CMV Forward: CGCAAATGGGCGGTAGGCGTG
  • 3'端测序 --
  • 载体标签 DD tag (N-端)
  • 载体抗性 卡那霉素
  • 稳定性 稳表达
  • 组成
  • 病毒/非病毒 腺病毒
pNTAP Shuttle A, 7735 bp                       version 016001

NOTE: The following sequence has been verified for accuracy
at the junctions. The remainder of the sequence has been 
obtained from existing data. 


    1  CATCATCAAT AATATACCTT ATTTTGGATT GAAGCCAATA TGATAATGAG
   51  GGGGTGGAGT TTGTGACGTG GCGCGGGGCG TGGGAACGGG GCGGGTGACG
  101  TAGTAGTGTG GCGGAAGTGT GATGTTGCAA GTGTGGCGGA ACACATGTAA
  151  GCGACGGATG TGGCAAAAGT GACGTTTTTG GTGTGCGCCG GTGTACACAG
  201  GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG TAAATTTGGG
  251  CGTAACCGAG TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA
  301  AGTGAAATCT GAATAATTTT GTGTTACTCA TAGCGCGTAA TACTGTAATA
  351  GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG
  401  TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC
  451  CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG
  501  GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT
  551  TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC
  601  AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG
  651  GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA
  701  TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC
  751  TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT
  801  TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC
  851  CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
  901  AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGAGATCTG CTAGCATGAA
  951  GCGACGATGG AAAAAGAATT TCATAGCCGT CTCAGCAGCC AACCGCTTTA
 1001  AGAAAATCTC ATCCTCCGGG GCACTTGGAA GCGGTAGCGG TACCATGGAC
 1051  GAGAAGACCA CCGGCTGGCG GGGCGGCCAC GTGGTGGAGG GCCTGGCCGG
 1101  CGAGCTGGAG CAGCTGCGGG CCAGGCTGGA GCACCACCCT CAGGGCCAGC
 1151  GGGAGCCCTC CGGCGGCTGC AAGCTGGGCG CCCGGGCGGA TCCCCCGGGC
 1201  GTCGACGCGG CCGCTCGAGC CGAAGCTTAT CGATACGCGT CCGATCGGAT
 1251  ATCCGATCCA CCGGATCTAG ATAACTGATC ATAATCAGCC ATACCACATT
 1301  TGTAGAGGTT TTACTTGCTT TAAAAAACCT CCCACACCTC CCCCTGAACC
 1351  TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT TATTGCAGCT
 1401  TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC
 1451  ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT
 1501  CTTAACGCGG ATCTGGGCGT GGTTAAGGGT GGGAAAGAAT ATATAAGGTG
 1551  GGGGTCTTAT GTAGTTTTGT ATCTGTTTTG CAGCAGCCGC CGCCGCCATG
 1601  AGCACCAACT CGTTTGATGG AAGCATTGTG AGCTCATATT TGACAACGCG
 1651  CATGCCCCCA TGGGCCGGGG TGCGTCAGAA TGTGATGGGC TCCAGCATTG
 1701  ATGGTCGCCC CGTCCTGCCC GCAAACTCTA CTACCTTGAC CTACGAGACC
 1751  GTGTCTGGAA CGCCGTTGGA GACTGCAGCC TCCGCCGCCG CTTCAGCCGC
 1801  TGCAGCCACC GCCCGCGGGA TTGTGACTGA CTTTGCTTTC CTGAGCCCGC
 1851  TTGCAAGCAG TGCAGCTTCC CGTTCATCCG CCCGCGATGA CAAGTTGACG
 1901  GCTCTTTTGG CACAATTGGA TTCTTTGACC CGGGAACTTA ATGTCGTTTC
 1951  TCAGCAGCTG TTGGATCTGC GCCAGCAGGT TTCTGCCCTG AAGGCTTCCT
 2001  CCCCTCCCAA TGCGGTTTAA AACATAAATA AAAAACCAGA CTCTGTTTGG
 2051  ATTTGGATCA AGCAAGTGTC TTGCTGTCTT TATTTAGGGG TTTTGCGCGC
 2101  GCGGTAGGCC CGGGACCAGC GGTCTCGGTC GTTGAGGGTC CTGTGTATTT
 2151  TTTCCAGGAC GTGGTAAAGG TGACTCTGGA TGTTCAGATA CATGGGCATA
 2201  AGCCCGTCTC TGGGGTGGAG GTAGCACCAC TGCAGAGCTT CATGCTGCGG
 2251  GGTGGTGTTG TAGATGATCC AGTCGTAGCA GGAGCGCTGG GCGTGGTGCC
 2301  TAAAAATGTC TTTCAGTAGC AAGCTGATTG CCAGGGGCAG GCCCTTGGTG
 2351  TAAGTGTTTA CAAAGCGGTT AAGCTGGGAT GGGTGCATAC GTGGGGATAT
 2401  GAGATGCATC TTGGACTGTA TTTTTAGGTT GGCTATGTTC CCAGCCATAT
 2451  CCCTCCGGGG ATTCATGTTG TGCAGAACCA CCAGCACAGT GTATCCGGTG
 2501  CACTTGGGAA ATTTGTCATG TAGCTTAGAA GGAAATGCGT GGAAGAACTT
 2551  GGAGACGCCC TTGTGACCTC CAAGATTTTC CATGCATTCG TCCATAATGA
 2601  TGGCAATGGG CCCACGGGCG GCGGCCTGGG CGAAGATATT TCTGGGATCA
 2651  CTAACGTCAT AGTTGTGTTC CAGGATGAGA TCGTCATAGG CCATTTTTAC
 2701  AAAGCGCGGG CGGAGGGTGC CAGACTGCGG TATAATGGTT CCATCCGGCC
 2751  CAGGGGCGTA GTTACCCTCA CAGATTTGCA TTTCCCACGC TTTGAGTTCA
 2801  GATGGGGGGA TCATGTCTAC CTGCGGGGCG ATGAAGAAAA CGGTTTCCGG
 2851  GGTAGGGGAG ATCAGCTGGG AAGAAAGCAG GTTCCTGAGC AGCTGCGACT
 2901  TACCGCAGCC GGTGGGCCCG TAAATCACAC CTATTACCGG GTGCAACTGG
 2951  TAGTTAAGAG AGCTGCAGCT GCCGTCATCC CTGAGCAGGG GGGCCACTTC
 3001  GTTAAGCATG TCCCTGACTC GCATGTTTTC CCTGACCAAA TCCGCCAGAA
 3051  GGCGCTCGCC GCCCAGCGAT AGCAGTTCTT GCAAGGAAGC AAAGTTTTTC
 3101  AACGGTTTGA GACCGTCCGC CGTAGGCATG CTTTTGAGCG TTTGACCAAG
 3151  CAGTTCCAGG CGGTCCCACA GCTCGGTCAC CTGCTCTACG GCATCTCGAT
 3201  CCAGCATATC TCCTCGTTTC GCGGGTTGGG GCGGCTTTCG CTGTACGGCA
 3251  GTAGTCGGTG CTCGTCCAGA CGGGCCAGGG TCATGTCTTT CCACGGGCGC
 3301  AGGGTCCTCG TCAGCGTAGT CTGGGTCACG GTGAAGGGGT GCGCTCCGGG
 3351  CTGCGCGCTG GCCAGGGTGC GCTTGAGGCT GGTCCTGCTG GTGCTGAAGC
 3401  GCTGCCGGTC TTCGCCCTGC GCGTCGGCCA GGTAGCATTT GACCATGGTG
 3451  TCATAGTCCA GCCCCTCCGC GGCGTGGCCC TTGGCGCGCA GCTTGCCCTT
 3501  GGAGGAGGCG CCGCACGAGG GGCAGTGCAG ACTTTTGAGG GCGTAGAGCT
 3551  TGGGCGCGAG AAATACCGAT TCCGGGGAGT AGGCATCCGC GCCGCAGGCC
 3601  CCGCAGACGG TCTCGCATTC CACGAGCCAG GTGAGCTCTG GCCGTTCGGG
 3651  GTCAAAAACC AGGTTTCCCC CATGCTTTTT GATGCGTTTC TTACCTCTGG
 3701  TTTCCATGAG CCGGTGTCCA CGCTCGGTGA CGAAAAGGCT GTCCGTGTCC
 3751  CCGTATACAG ACTTGAGAGG GAGTTTAAAC GAATTCAATA GCTTGTTGCA
 3801  TGGGCGGCGA TATAAAATGC AAGGTGCTGC TCAAAAAATC AGGCAAAGCC
 3851  TCGCGCAAAA AAGAAAGCAC ATCGTAGTCA TGCTCATGCA GATAAAGGCA
 3901  GGTAAGCTCC GGAACCACCA CAGAAAAAGA CACCATTTTT CTCTCAAACA
 3951  TGTCTGCGGG TTTCTGCATA AACACAAAAT AAAATAACAA AAAAACATTT
 4001  AAACATTAGA AGCCTGTCTT ACAACAGGAA AAACAACCCT TATAAGCATA
 4051  AGACGGACTA CGGCCATGCC GGCGTGACCG TAAAAAAACT GGTCACCGTG
 4101  ATTAAAAAGC ACCACCGACA GCTCCTCGGT CATGTCCGGA GTCATAATGT
 4151  AAGACTCGGT AAACACATCA GGTTGATTCA CATCGGTCAG TGCTAAAAAG
 4201  CGACCGAAAT AGCCCGGGGG AATACATACC CGCAGGCGTA GAGACAACAT
 4251  TACAGCCCCC ATAGGAGGTA TAACAAAATT AATAGGAGAG AAAAACACAT
 4301  AAACACCTGA AAAACCCTCC TGCCTAGGCA AAATAGCACC CTCCCGCTCC
 4351  AGAACAACAT ACAGCGCTTC CACAGCGGCA GCCATAACAG TCAGCCTTAC
 4401  CAGTAAAAAA GAAAACCTAT TAAAAAAACA CCACTCGACA CGGCACCAGC
 4451  TCAATCAGTC ACAGTGTAAA AAAGGGCCAA GTGCAGAGCG AGTATATATA
 4501  GGACTAAAAA ATGACGTAAC GGTTAAAGTC CACAAAAAAC ACCCAGAAAA
 4551  CCGCACGCGA ACCTACGCCC AGAAACGAAA GCCAAAAAAC CCACAACTTC
 4601  CTCAAATCGT CACTTCCGTT TTCCCACGTT ACGTAACTTC CCATTTTAAG
 4651  AAAACTACAA TTCCCAACAC ATACAAGTTA CTCCGCCCTA AAACCTACGT
 4701  CACCCGCCCC GTTCCCACGC CCCGCGCCAC GTCACAAACT CCACCCCCTC
 4751  ATTATCATAT TGGCTTCAAT CCAAAATAAG GTATATTATT GATGATGTTA
 4801  ATTAACATGC ATGGATCCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA
 4851  AGGAGAAAAT ACCGCATCAG GCGCTCTTCC GCTTCCTCGC TCACTGACTC
 4901  GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG
 4951  CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG
 5001  TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT
 5051  GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC
 5101  GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG
 5151  TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
 5201  TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC
 5251  ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
 5301  CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC
 5351  CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC
 5401  TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT
 5451  GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC
 5501  AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG
 5551  TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT
 5601  TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA
 5651  TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC
 5701  GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC
 5751  CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
 5801  AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG
 5851  CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
 5901  ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT
 5951  ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC
 6001  CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC
 6051  ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
 6101  TAATAGTTTG CGCAACGTTG TTGCCATTGC TGCAGCCATG AGATTATCAA
 6151  AAAGGATCTT CACCTAGATC CTTTTCACGT AGAAAGCCAG TCCGCAGAAA
 6201  CGGTGCTGAC CCCGGATGAA TGTCAGCTAC TGGGCTATCT GGACAAGGGA
 6251  AAACGCAAGC GCAAAGAGAA AGCAGGTAGC TTGCAGTGGG CTTACATGGC
 6301  GATAGCTAGA CTGGGCGGTT TTATGGACAG CAAGCGAACC GGAATTGCCA
 6351  GCTGGGGCGC CCTCTGGTAA GGTTGGGAAG CCCTGCAAAG TAAACTGGAT
 6401  GGCTTTCTTG CCGCCAAGGA TCTGATGGCG CAGGGGATCA AGCTCTGATC
 6451  AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC
 6501  GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC
 6551  ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC
 6601  AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
 6651  GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT
 6701  TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC
 6751  TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT
 6801  CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC
 6851  GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG
 6901  AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
 6951  GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA
 7001  GGCGAGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT
 7051  GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC
 7101  TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC
 7151  CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG
 7201  TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
 7251  CTTCTTGACG AGTTCTTCTG AATTTTGTTA AAATTTTTGT TAAATCAGCT
 7301  CATTTTTTAA CCAATAGGCC GAAATCGGCA CCATCCCTTA TAAATCAAAA
 7351  GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC
 7401  ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC
 7451  AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGTGG
 7501  TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT
 7551  TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA
 7601  AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC GGTCACGCTG
 7651  CGCGTAACCA CCACACCCGC GCGCTTAATG CGCCGCTACA GGGCGCGTCC
 7701  ATTCGCCATT CAGGATCGAA TTAATTCTTA ATTAA
科研狗©2015-2024 科研好助手,京ICP备20005780号-1 建议意见

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