pNTAP-Shuttle-B

创建日期:2013-08-05 查看:117
  • 出品公司 Agilent
  • 其他名称
  • 载体类型 腺病毒载体
  • 表达水平
  • 克隆方法 限制性内切酶,多克隆位点
  • 载体大小 7736 bp
  • 5'端测序 CMV Forward: CGCAAATGGGCGGTAGGCGTG
  • 3'端测序 --
  • 载体标签 SBP ; CBP (N-端)
  • 载体抗性 卡那霉素
  • 稳定性 稳表达
  • 组成
  • 病毒/非病毒 腺病毒
pNTAP Shuttle B, 7736 bp                       version 016001

NOTE: The following sequence has been verified for accuracy
at the junctions. The remainder of the sequence has been 
obtained from existing data. 


    1  CATCATCAAT AATATACCTT ATTTTGGATT GAAGCCAATA TGATAATGAG
   51  GGGGTGGAGT TTGTGACGTG GCGCGGGGCG TGGGAACGGG GCGGGTGACG
  101  TAGTAGTGTG GCGGAAGTGT GATGTTGCAA GTGTGGCGGA ACACATGTAA
  151  GCGACGGATG TGGCAAAAGT GACGTTTTTG GTGTGCGCCG GTGTACACAG
  201  GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG TAAATTTGGG
  251  CGTAACCGAG TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA
  301  AGTGAAATCT GAATAATTTT GTGTTACTCA TAGCGCGTAA TACTGTAATA
  351  GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG
  401  TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC
  451  CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG
  501  GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT
  551  TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC
  601  AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG
  651  GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA
  701  TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA GCGGTTTGAC
  751  TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT
  801  TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC
  851  CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
  901  AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGAGATCTG CTAGCATGAA
  951  GCGACGATGG AAAAAGAATT TCATAGCCGT CTCAGCAGCC AACCGCTTTA
 1001  AGAAAATCTC ATCCTCCGGG GCACTTGGAA GCGGTAGCGG TACCATGGAC
 1051  GAGAAGACCA CCGGCTGGCG GGGCGGCCAC GTGGTGGAGG GCCTGGCCGG
 1101  CGAGCTGGAG CAGCTGCGGG CCAGGCTGGA GCACCACCCT CAGGGCCAGC
 1151  GGGAGCCCTC CGGCGGCTGC AAGCTGGGCT GCCCGGGCGG ATCCCCCGGG
 1201  CGTCGACGCG GCCGCTCGAG CCGAAGCTTA TCGATACGCG TCCGATCGGA
 1251  TATCCGATCC ACCGGATCTA GATAACTGAT CATAATCAGC CATACCACAT
 1301  TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC
 1351  CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC
 1401  TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG
 1451  CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA
 1501  TCTTAACGCG GATCTGGGCG TGGTTAAGGG TGGGAAAGAA TATATAAGGT
 1551  GGGGGTCTTA TGTAGTTTTG TATCTGTTTT GCAGCAGCCG CCGCCGCCAT
 1601  GAGCACCAAC TCGTTTGATG GAAGCATTGT GAGCTCATAT TTGACAACGC
 1651  GCATGCCCCC ATGGGCCGGG GTGCGTCAGA ATGTGATGGG CTCCAGCATT
 1701  GATGGTCGCC CCGTCCTGCC CGCAAACTCT ACTACCTTGA CCTACGAGAC
 1751  CGTGTCTGGA ACGCCGTTGG AGACTGCAGC CTCCGCCGCC GCTTCAGCCG
 1801  CTGCAGCCAC CGCCCGCGGG ATTGTGACTG ACTTTGCTTT CCTGAGCCCG
 1851  CTTGCAAGCA GTGCAGCTTC CCGTTCATCC GCCCGCGATG ACAAGTTGAC
 1901  GGCTCTTTTG GCACAATTGG ATTCTTTGAC CCGGGAACTT AATGTCGTTT
 1951  CTCAGCAGCT GTTGGATCTG CGCCAGCAGG TTTCTGCCCT GAAGGCTTCC
 2001  TCCCCTCCCA ATGCGGTTTA AAACATAAAT AAAAAACCAG ACTCTGTTTG
 2051  GATTTGGATC AAGCAAGTGT CTTGCTGTCT TTATTTAGGG GTTTTGCGCG
 2101  CGCGGTAGGC CCGGGACCAG CGGTCTCGGT CGTTGAGGGT CCTGTGTATT
 2151  TTTTCCAGGA CGTGGTAAAG GTGACTCTGG ATGTTCAGAT ACATGGGCAT
 2201  AAGCCCGTCT CTGGGGTGGA GGTAGCACCA CTGCAGAGCT TCATGCTGCG
 2251  GGGTGGTGTT GTAGATGATC CAGTCGTAGC AGGAGCGCTG GGCGTGGTGC
 2301  CTAAAAATGT CTTTCAGTAG CAAGCTGATT GCCAGGGGCA GGCCCTTGGT
 2351  GTAAGTGTTT ACAAAGCGGT TAAGCTGGGA TGGGTGCATA CGTGGGGATA
 2401  TGAGATGCAT CTTGGACTGT ATTTTTAGGT TGGCTATGTT CCCAGCCATA
 2451  TCCCTCCGGG GATTCATGTT GTGCAGAACC ACCAGCACAG TGTATCCGGT
 2501  GCACTTGGGA AATTTGTCAT GTAGCTTAGA AGGAAATGCG TGGAAGAACT
 2551  TGGAGACGCC CTTGTGACCT CCAAGATTTT CCATGCATTC GTCCATAATG
 2601  ATGGCAATGG GCCCACGGGC GGCGGCCTGG GCGAAGATAT TTCTGGGATC
 2651  ACTAACGTCA TAGTTGTGTT CCAGGATGAG ATCGTCATAG GCCATTTTTA
 2701  CAAAGCGCGG GCGGAGGGTG CCAGACTGCG GTATAATGGT TCCATCCGGC
 2751  CCAGGGGCGT AGTTACCCTC ACAGATTTGC ATTTCCCACG CTTTGAGTTC
 2801  AGATGGGGGG ATCATGTCTA CCTGCGGGGC GATGAAGAAA ACGGTTTCCG
 2851  GGGTAGGGGA GATCAGCTGG GAAGAAAGCA GGTTCCTGAG CAGCTGCGAC
 2901  TTACCGCAGC CGGTGGGCCC GTAAATCACA CCTATTACCG GGTGCAACTG
 2951  GTAGTTAAGA GAGCTGCAGC TGCCGTCATC CCTGAGCAGG GGGGCCACTT
 3001  CGTTAAGCAT GTCCCTGACT CGCATGTTTT CCCTGACCAA ATCCGCCAGA
 3051  AGGCGCTCGC CGCCCAGCGA TAGCAGTTCT TGCAAGGAAG CAAAGTTTTT
 3101  CAACGGTTTG AGACCGTCCG CCGTAGGCAT GCTTTTGAGC GTTTGACCAA
 3151  GCAGTTCCAG GCGGTCCCAC AGCTCGGTCA CCTGCTCTAC GGCATCTCGA
 3201  TCCAGCATAT CTCCTCGTTT CGCGGGTTGG GGCGGCTTTC GCTGTACGGC
 3251  AGTAGTCGGT GCTCGTCCAG ACGGGCCAGG GTCATGTCTT TCCACGGGCG
 3301  CAGGGTCCTC GTCAGCGTAG TCTGGGTCAC GGTGAAGGGG TGCGCTCCGG
 3351  GCTGCGCGCT GGCCAGGGTG CGCTTGAGGC TGGTCCTGCT GGTGCTGAAG
 3401  CGCTGCCGGT CTTCGCCCTG CGCGTCGGCC AGGTAGCATT TGACCATGGT
 3451  GTCATAGTCC AGCCCCTCCG CGGCGTGGCC CTTGGCGCGC AGCTTGCCCT
 3501  TGGAGGAGGC GCCGCACGAG GGGCAGTGCA GACTTTTGAG GGCGTAGAGC
 3551  TTGGGCGCGA GAAATACCGA TTCCGGGGAG TAGGCATCCG CGCCGCAGGC
 3601  CCCGCAGACG GTCTCGCATT CCACGAGCCA GGTGAGCTCT GGCCGTTCGG
 3651  GGTCAAAAAC CAGGTTTCCC CCATGCTTTT TGATGCGTTT CTTACCTCTG
 3701  GTTTCCATGA GCCGGTGTCC ACGCTCGGTG ACGAAAAGGC TGTCCGTGTC
 3751  CCCGTATACA GACTTGAGAG GGAGTTTAAA CGAATTCAAT AGCTTGTTGC
 3801  ATGGGCGGCG ATATAAAATG CAAGGTGCTG CTCAAAAAAT CAGGCAAAGC
 3851  CTCGCGCAAA AAAGAAAGCA CATCGTAGTC ATGCTCATGC AGATAAAGGC
 3901  AGGTAAGCTC CGGAACCACC ACAGAAAAAG ACACCATTTT TCTCTCAAAC
 3951  ATGTCTGCGG GTTTCTGCAT AAACACAAAA TAAAATAACA AAAAAACATT
 4001  TAAACATTAG AAGCCTGTCT TACAACAGGA AAAACAACCC TTATAAGCAT
 4051  AAGACGGACT ACGGCCATGC CGGCGTGACC GTAAAAAAAC TGGTCACCGT
 4101  GATTAAAAAG CACCACCGAC AGCTCCTCGG TCATGTCCGG AGTCATAATG
 4151  TAAGACTCGG TAAACACATC AGGTTGATTC ACATCGGTCA GTGCTAAAAA
 4201  GCGACCGAAA TAGCCCGGGG GAATACATAC CCGCAGGCGT AGAGACAACA
 4251  TTACAGCCCC CATAGGAGGT ATAACAAAAT TAATAGGAGA GAAAAACACA
 4301  TAAACACCTG AAAAACCCTC CTGCCTAGGC AAAATAGCAC CCTCCCGCTC
 4351  CAGAACAACA TACAGCGCTT CCACAGCGGC AGCCATAACA GTCAGCCTTA
 4401  CCAGTAAAAA AGAAAACCTA TTAAAAAAAC ACCACTCGAC ACGGCACCAG
 4451  CTCAATCAGT CACAGTGTAA AAAAGGGCCA AGTGCAGAGC GAGTATATAT
 4501  AGGACTAAAA AATGACGTAA CGGTTAAAGT CCACAAAAAA CACCCAGAAA
 4551  ACCGCACGCG AACCTACGCC CAGAAACGAA AGCCAAAAAA CCCACAACTT
 4601  CCTCAAATCG TCACTTCCGT TTTCCCACGT TACGTCACTT CCCATTTTAA
 4651  GAAAACTACA ATTCCCAACA CATACAAGTT ACTCCGCCCT AAAACCTACG
 4701  TCACCCGCCC CGTTCCCACG CCCCGCGCCA CGTCACAAAC TCCACCCCCT
 4751  CATTATCATA TTGGCTTCAA TCCAAAATAA GGTATATTAT TGATGATGTT
 4801  AATTAACATG CATGGATCCA TATGCGGTGT GAAATACCGC ACAGATGCGT
 4851  AAGGAGAAAA TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT
 4901  CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG
 4951  GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT
 5001  GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC
 5051  TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
 5101  CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
 5151  GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC
 5201  TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT
 5251  CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
 5301  GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT
 5351  CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
 5401  CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
 5451  TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA
 5501  CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA
 5551  GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
 5601  TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
 5651  ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
 5701  CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
 5751  CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT
 5801  AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
 5851  GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA
 5901  GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA
 5951  TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
 6001  CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
 6051  CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG
 6101  TTAATAGTTT GCGCAACGTT GTTGCCATTG CTGCAGCCAT GAGATTATCA
 6151  AAAAGGATCT TCACCTAGAT CCTTTTCACG TAGAAAGCCA GTCCGCAGAA
 6201  ACGGTGCTGA CCCCGGATGA ATGTCAGCTA CTGGGCTATC TGGACAAGGG
 6251  AAAACGCAAG CGCAAAGAGA AAGCAGGTAG CTTGCAGTGG GCTTACATGG
 6301  CGATAGCTAG ACTGGGCGGT TTTATGGACA GCAAGCGAAC CGGAATTGCC
 6351  AGCTGGGGCG CCCTCTGGTA AGGTTGGGAA GCCCTGCAAA GTAAACTGGA
 6401  TGGCTTTCTT GCCGCCAAGG ATCTGATGGC GCAGGGGATC AAGCTCTGAT
 6451  CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA
 6501  CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG
 6551  CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG
 6601  CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
 6651  TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG
 6701  TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG
 6751  CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC
 6801  TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA
 6851  CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC
 6901  GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
 6951  GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA
 7001  AGGCGAGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC
 7051  TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA
 7101  CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA
 7151  CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC
 7201  GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
 7251  CCTTCTTGAC GAGTTCTTCT GAATTTTGTT AAAATTTTTG TTAAATCAGC
 7301  TCATTTTTTA ACCAATAGGC CGAAATCGGC ACCATCCCTT ATAAATCAAA
 7351  AGAATAGACC GAGATAGGGT TGAGTGTTGT TCCAGTTTGG AACAAGAGTC
 7401  CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT
 7451  CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGTG
 7501  GTCGAGGTGC CGTAAAGCAC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT
 7551  TTAGAGCTTG ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG
 7601  AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG GCAAGTGTAG CGGTCACGCT
 7651  GCGCGTAACC ACCACACCCG CGCGCTTAAT GCGCCGCTAC AGGGCGCGTC
 7701  CATTCGCCAT TCAGGATCGA ATTAATTCTT AATTAA
科研狗©2015-2024 科研好助手,京ICP备20005780号-1 建议意见

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