pDC312

创建日期:2013-09-13 查看:126
  • 出品公司 Microbix Biosystems
  • 其他名称
  • 载体类型 腺病毒表达载体
  • 表达水平
  • 克隆方法 --
  • 载体大小 3287 bp
  • 5'端测序 --
  • 3'端测序 --
  • 载体标签 --
  • 载体抗性 氨苄青霉素(Ampicillin)
  • 稳定性 --
  • 组成 组成型
  • 病毒/非病毒 腺病毒
LOCUS       pDC312       3287 bp dsDNA    Circular
DEFINITION  Puc based lox shuttle plasmid  for Cre-based Ad vector rescue Cummings & Graham, unpublished
COMMENT     This file is best viewed with a free Vector NTI Viewer:
COMMENT     http://www.informaxinc.com/view/viewer.html
COMMENT     VNTREPLTYPE|Plasmid|
COMMENT     VNTEXTCHREPL|Bacteria|
FEATURES               Location/Qualifiers
     misc_feature    525..558
                     /label=loxP
     rep_origin      977..1277
                     /label=ori
     misc_feature    2828..453
                     /label=Ad
     CDS             complement(1727..2543)
                     /label=amp
     CDS             3197..1
                     /label=ITR
     CDS             complement(2..102)
                     /label=ITR
ORIGIN
        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 
       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 
      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 
      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 
      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 
      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg 
      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc 
      421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gtcgacgagc tcaagcttag 
      481 atctgctagc aggcctgaat tcggatcctc gactcgaaga tccaataact tcgtatagca 
      541 tacattatac gaagttataa gtgccgccct ataccttgtc tgcctccccg cgttgcgtcg 
      601 cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga 
      661 ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc 
      721 aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc 
      781 tcggctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 
      841 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 
      901 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 
      961 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 
     1021 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 
     1081 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 
     1141 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 
     1201 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 
     1261 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 
     1321 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 
     1381 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 
     1441 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 
     1501 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 
     1561 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 
     1621 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 
     1681 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 
     1741 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 
     1801 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 
     1861 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 
     1921 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 
     1981 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 
     2041 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 
     2101 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 
     2161 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 
     2221 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 
     2281 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 
     2341 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg 
     2401 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 
     2461 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 
     2521 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 
     2581 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 
     2641 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 
     2701 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 
     2761 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 
     2821 tatcactcta ggcaaaatag caccctcccg ctccagaaca acatacagcg cttcacagcg 
     2881 gcagcctaac agtcagcctt accagtaaaa aagaaaacct attaaaaaaa caccactcga 
     2941 cacggcacca gctcaatcag tcacagtgta aaaaagggcc aagtgcagag cgagtatata 
     3001 taggactaaa aaatgacgta acggttaaag tccacaaaaa acacccagaa aaccgcacgc 
     3061 gaacctacgc ccagaaacga aagccaaaaa acccacaact tcctcaaatc gtcacttccg 
     3121 ttttcccacg ttacgtaact tcccatttta agaaaactac aattcccaac acatacaagt 
     3181 tactccgccc taaaacctac gtcacccgcc ccgttcccac gccccgcgcc acgtcacaaa 
     3241 ctccaccccc tcattatcat attggcttca atccaaaata aggtata 
//
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