LOCUS       pDC315       3913 bp dsDNA    Circular
DEFINITION  Puc based lox HCMV shuttle plasmid  for Cre-based Ad vector rescue Cummings & Graham, unpublished
COMMENT     This file is best viewed with a free Vector NTI Viewer:
COMMENT     VNTREPLTYPE|Plasmid|
COMMENT     VNTEXTCHREPL|Bacteria|
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES               Location/Qualifiers
     promoter        469..991
                     /note="MCMV promoter - Short"
                     /label=MCMV
     misc_feature    1190..1223
                     /label=loxP
     polyA_signal    1029..1188
                     /label=SV40\PolyA
     rep_origin      1603..1903
                     /label=ori
     CDS             complement(2353..3169)
                     /label=amp
     misc_feature    3454..453
                     /label=Ad
     CDS             3823..1
                     /label=ITR
     CDS             complement(2..102)
                     /label=ITR
ORIGIN
        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt 
       61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 
      121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 
      181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 
      241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 
      301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg 
      361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc 
      421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gatatactga gtcattaggg 
      481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc 
      541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa 
      601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa 
      661 taggggtgag tcattgggtt tttccagcca tttaattaaa acgccatgta ctttcccacc 
      721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat 
      781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg 
      841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cactcattct 
      901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca 
      961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcaa gctgctagca aggatccagc 
     1021 ttgtcgactt cgagcaactt gtttattgca gcttataatg gttacaaata aagcaatagc 
     1081 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 
     1141 ctcatcaatg tatcttatca tgtctggatc gtctagcatc gaagatccaa taacttcgta 
     1201 tagcatacat tatacgaagt tataagtagc ttggcgtaat catggtcata gctgtttcct 
     1261 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt 
     1321 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc 
     1381 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg 
     1441 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 
     1501 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 
     1561 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 
     1621 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 
     1681 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 
     1741 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 
     1801 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat 
     1861 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 
     1921 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 
     1981 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 
     2041 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 
     2101 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 
     2161 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 
     2221 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 
     2281 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 
     2341 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 
     2401 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 
     2461 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 
     2521 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 
     2581 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 
     2641 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 
     2701 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 
     2761 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 
     2821 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 
     2881 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 
     2941 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 
     3001 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa 
     3061 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 
     3121 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 
     3181 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 
     3241 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 
     3301 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 
     3361 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 
     3421 atgacattaa cctataaaaa taggcgtatc actctaggca aaatagcacc ctcccgctcc 
     3481 agaacaacat acagcgcttc acagcggcag cctaacagtc agccttacca gtaaaaaaga 
     3541 aaacctatta aaaaaacacc actcgacacg gcaccagctc aatcagtcac agtgtaaaaa 
     3601 agggccaagt gcagagcgag tatatatagg actaaaaaat gacgtaacgg ttaaagtcca 
     3661 caaaaaacac ccagaaaacc gcacgcgaac ctacgcccag aaacgaaagc caaaaaaccc 
     3721 acaacttcct caaatcgtca cttccgtttt cccacgttac gtaacttccc attttaagaa 
     3781 aactacaatt cccaacacat acaagttact ccgccctaaa acctacgtca cccgccccgt 
     3841 tcccacgccc cgcgccacgt cacaaactcc accccctcat tatcatattg gcttcaatcc 
     3901 aaaataaggt ata 
//