服务热线
178 0020 3020
LOCUS pLVX-rHom-1 8848 bp DNA circular 10-NOV-2011 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Catalog Nos. 635077, 635076 FEATURES Location/Qualifiers LTR 1..635 /label=5'\LTR misc_feature 685..822 /label=packaging\signal misc_feature 2028..2151 /label=CTS/cppt\(DNA\flap) promoter 2185..2788 /label=pCMV misc_feature 4770..5361 /label=WPRE rep_origin complement(6671..7341) /label=pUC\origin CDS complement(7486..8482) /label=Amp(R) misc_binding 1303..1536 /label=RRE LTR 5565..6201 /label=3'\LTR misc_feature 3549..4124 /label=IRES CDS 4157..4756 /label=Puro(R) CDS 2849..3172 /label=DmrD CDS 3176..3499 /label=DmrD BASE COUNT 2267 a 2210 c 2294 g 2077 t ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tagctagagg atctatttcc ggtgaattcc tcgagactag 2821 tatgccagaa gccgccacca tggcttctag aggagtgcag gtggagacta tctccccagg 2881 agacgggcgc accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct 2941 tgaagatgga aagaaaatgg attcctcccg ggacagaaac aagcccttta agtttatgct 3001 aggcaagcag gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca 3061 gagagccaaa ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat 3121 catcccacca catgccactc tcgtcttcga tgtggagctt ctaaaactgg aaactagagg 3181 agtgcaggtg gagactatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac 3241 ctgcgtggtg cactacaccg ggatgcttga agatggaaag aaaatggatt cctcccggga 3301 cagaaacaag ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga 3361 aggggttgcc cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc 3421 ctatggtgcc actgggcacc caggcatcat cccaccacat gccactctcg tcttcgatgt 3481 ggagcttcta aaactggaaa ctagttatcc gtacgacgta ccaagcggcc gcggatccta 3541 cgtatgaccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat 3601 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg 3661 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc 3721 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt 3781 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg 3841 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac 3901 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg 3961 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg 4021 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc 4081 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaacccac 4141 aaggagacga ccttccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga 4201 cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca 4261 caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac 4321 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt 4381 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat 4441 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc 4501 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca 4561 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg 4621 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg 4681 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg 4741 caagcccggt gcctagacgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa 4801 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 4861 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 4921 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 4981 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 5041 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 5101 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 5161 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 5221 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 5281 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 5341 tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc 5401 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga 5461 ggaggaggag gtgggttttt ccagtcacac ctcaggtacc tttaagacca atgacttaca 5521 aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc 5581 actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc 5641 ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt 5701 gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca 5761 ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag 5821 agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt 5881 acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag 5941 ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag 6001 ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 6061 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 6121 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 6181 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 6241 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt taccgtcgac 6301 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 6361 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 6421 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 6481 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 6541 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 6601 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 6661 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 6721 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 6781 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 6841 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 6901 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 6961 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 7021 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 7081 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 7141 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa 7201 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 7261 tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 7321 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 7381 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 7441 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 7501 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 7561 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 7621 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 7681 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 7741 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 7801 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 7861 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 7921 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 7981 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 8041 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 8101 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 8161 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 8221 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 8281 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 8341 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 8401 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 8461 ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata 8521 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 8581 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga 8641 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca 8701 attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa 8761 agaaattgta tttgttaaat atgtactaca aacttagtag tttttaaaga aattgtattt 8821 gttaaatatg tactacaaac ttagtagt //