LOCUS       pLVX-Tet-On Advanced	11625 bp 	DNA	SYN
DEFINITION  pLVX-Tet-On Advanced
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..11625
                     /organism="pLVX-Tet-On Advanced"
                     /mol_type="other DNA"
     misc_feature    454..634
                     /label="HIV-1_5_LTR"
     misc_feature    454..634
                     /label="truncHIV-1_3_LTR"
     misc_feature    745..789
                     /label="HIV-1_psi_pack"
     CDS             complement(1015..1476)
                     /label="ORF frame 1"

     CDS             1181..2032
                     /label="ORF frame 2"

     misc_feature    1303..1536
                     /label="RRE"

     misc_feature    2031..2046
                     /label="cPPT"

     promoter        2198..2702
                     /label="CMV_immearly_promoter"

     misc_feature    2205..2492
                     /label="CAG_enhancer"

     misc_feature    2659..2679
                     /label="CMV_fwd_primer"

     promoter        2660..2729
                     /label="CMV_promoter"

     misc_feature    2703..2722
                     /label="pCEP_fwd_primer"

     misc_feature    2705..2729
                     /label="LNCX_primer"

     CDS             2797..3543
                     /label="ORF frame 1"

     misc_feature    3581..4132
                     /label="IRES"

     CDS             4157..4951
                     /label="ORF frame 2"

     gene            4172..4948
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"

     misc_feature    5149..5724
                     /label="WPRE"

     CDS             5237..5764
                     /label="ORF frame 2"

     misc_feature    6395..6575
                     /label="truncHIV-1_3_LTR"

     misc_feature    6395..6575
                     /label="HIV-1_5_LTR"

     misc_feature    complement(6682..6699)
                     /label="BGH_rev_primer"

     terminator      6685..6912
                     /label="bGH_PA_terminator"

     rep_origin      6975..7281
                     /label="f1_origin"

     misc_feature    complement(7395..7415)

     misc_feature    complement(7401..7616)
                     /label="SV40_enhancer"

     promoter        7413..7681
                     /label="SV40_promoter"

     rep_origin      7580..7657
                     /label="SV40_origin"

     misc_feature    7642..7661
                     /label="SV40pro_F_primer"

     CDS             complement(7715..8536)
                     /label="ORF frame 3"

     CDS             7778..8803
                     /label="ORF frame 2"

     gene            7793..8800
                     /label="hygroB"
                     /gene="hygroB"

     terminator      8936..9055
                     /label="SV40_PA_terminator"

     misc_feature    9024..9043
                     /label="EBV_rev_primer"

     promoter        complement(9099..9117)
                     /label="M13_reverse_primer"

     misc_feature    complement(9116..9138)
                     /label="M13_pUC_rev_primer"

     promoter        complement(9152..9181)
                     /label="lac_promoter"

     gene            complement(10264..11124)
                     /label="Ampicillin"
                     /gene="Ampicillin"

     CDS             complement(10264..11124)
                     /label="ORF frame 1"

     promoter        complement(11166..11194)
                     /label="AmpR_promoter"

     terminator      11280..11411
                     /label="SV40_PA_terminator"

     misc_feature    11368..11387
                     /label="EBV_rev_primer"

ORIGIN
    1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
   61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
  121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
  181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
  241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
  301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
  361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
  421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
  481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
  541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
  601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
  661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
  721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
  781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
  841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
  901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
  961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
 2761 tccatagaag acaccgactc tactagagga tccaccatgt ctagactgga caagagcaaa
 2821 gtcataaacg gcgctctgga attactcaat ggagtcggta tcgaaggcct gacgacaagg
 2881 aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag
 2941 cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc
 3001 cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc attccgctgt
 3061 gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa
 3121 cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag
 3181 aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa
 3241 caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca
 3301 cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc
 3361 ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg
 3421 gccgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt
 3481 gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg
 3541 taactagaat tccgcccccc cccccccccc ccctctccct cccccccccc taacgttact
 3601 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
 3661 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
 3721 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
 3781 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
 3841 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
 3901 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
 3961 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
 4021 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
 4081 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
 4141 taagcttgcc acaaccatgg ctgaacaaga tggattgcac gcaggttctc cggccgcttg
 4201 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
 4261 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
 4321 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
 4381 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
 4441 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
 4501 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
 4561 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
 4621 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
 4681 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
 4741 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
 4801 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
 4861 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
 4921 cttctatcgc cttcttgacg agttcttctg agcgggatcg gctagagtcg acccggacgg
 4981 gacgctcctg cgcctgatac agaacgaatt gcttgcaggc atctcatgag tgtgtcttcc
 5041 cgttttccgc ctgaggtcac tgcgtggatg gagcgctggc gcctgctgcg cgacggcgag
 5101 ctgctcacca cccactcgag tctagagggc ccgacgcgtc tggaacaatc aacctctgga
 5161 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
 5221 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
 5281 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
 5341 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
 5401 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
 5461 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
 5521 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
 5581 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
 5641 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
 5701 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac
 5761 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
 5821 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
 5881 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga
 5941 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
 6001 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
 6061 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
 6121 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
 6181 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
 6241 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
 6301 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
 6361 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
 6421 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
 6481 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
 6541 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
 6601 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta
 6661 gcgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
 6721 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
 6781 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
 6841 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
 6901 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
 6961 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
 7021 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
 7081 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
 7141 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
 7201 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
 7261 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
 7321 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
 7381 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
 7441 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
 7501 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
 7561 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
 7621 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
 7681 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
 7741 agcttgtata tccattttcg gatctgatca gcacgtgatg aaaaagcctg aactcaccgc
 7801 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
 7861 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
 7921 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
 7981 atcggccgcg ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac
 8041 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
 8101 gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag
 8161 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
 8221 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
 8281 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
 8341 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
 8401 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
 8461 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
 8521 cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg
 8581 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
 8641 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
 8701 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
 8761 tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagcacgtgc tacgagattt
 8821 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
 8881 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
 8941 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
 9001 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
 9061 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
 9121 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
 9181 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
 9241 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
 9301 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
 9361 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
 9421 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
 9481 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
 9541 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
 9601 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
 9661 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
 9721 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
 9781 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
 9841 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
 9901 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
 9961 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
10021 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
10081 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
10141 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
10201 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
10261 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
10321 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
10381 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
10441 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
10501 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
10561 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
10621 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
10681 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
10741 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
10801 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
10861 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
10921 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
10981 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
11041 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
11101 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
11161 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
11221 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact
11281 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
11341 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
11401 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
11461 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
11521 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttt
11581 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
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