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LOCUS pLVX-DD-ZsGreen1_Control 9087 bp DNA circular 6-NOV-2009 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Cat. No. 631751,631752 FEATURES Location/Qualifiers LTR 1..635 /label=5'\LTR misc_feature 685..822 /label=packaging\signal promoter 2185..2787 /label=CMV\IE\Promoter misc_feature 5055..5646 /label=WPRE rep_origin complement(6955..7628) /label=pUC\origin CDS complement(7773..8769) /label=Amp(R) misc_binding 1303..1536 /label=RRE misc_binding 636..653 /label=PBS misc_feature 2028..2151 /label=cPPT/CTS LTR 5849..6485 /label=3'\LTR misc_feature 3913..4421 /label=PGK\Promoter misc_feature 4442..5041 /label=Puro(R) CDS 2880..3203 /label=DD CDS 3210..3902 /label=ZsGreen1 BASE COUNT 2245 a 2363 c 2407 g 2072 t ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatcccgc gactctagaa 2881 tgggagtgca ggtggaaacc atctccccag gagacgggcg caccttcccc aagcgcggcc 2941 agacctgtgt ggtgcactac accgggatgc ttgaagatgg aaagaaagtc gattcctccc 3001 gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc cgaggctggg 3061 aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata tctccagatt 3121 atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact ctcgtcttcg 3181 atgtggagct tctaaaaccg gaaagatcta tggcccagtc caagcacggc ctgaccaagg 3241 agatgaccat gaagtaccgc atggagggct gcgtggacgg ccacaagttc gtgatcaccg 3301 gcgagggcat cggctacccc ttcaagggca agcaggccat caacctgtgc gtggtggagg 3361 gcggcccctt gcccttcgcc gaggacatct tgtctgccgc cttcatgtac ggcaaccgcg 3421 tgttcaccga gtatccccag gacatcgtcg actacttcaa gaactcctgc cccgccggct 3481 acacctggga ccgctccttc ctgttcgagg acggcgccgt gtgcatctgc aacgccgaca 3541 tcaccgtgag cgtggaggag aactgcatgt accacgagtc caagttctac ggcgtgaact 3601 tccccgccga cggccccgtg atgaagaaga tgaccgacaa ctgggagccc tcctgcgaga 3661 agatcatccc cgtgcccaag cagggcatct tgaagggcga cgtgagcatg tacctgctgc 3721 tgaaggacgg tggccgcttg cgctgccagt tcgacaccgt gtacaaggcc aagtccgtgc 3781 cccgcaagat gcccgactgg cacttcatcc agcacaagct gacccgcgag gaccgcagcg 3841 acgccaagaa ccagaagtgg cacctgaccg agcacgccat cgcctccggc tccgccttgc 3901 cctagtctag ataattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat 3961 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac 4021 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac 4081 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga 4141 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc 4201 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct 4261 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg 4321 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt 4381 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac 4441 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt 4501 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga 4561 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga 4621 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga 4681 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg 4741 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga 4801 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg 4861 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct 4921 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc 4981 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg 5041 accgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 5101 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 5161 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 5221 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 5281 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 5341 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 5401 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 5461 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 5521 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 5581 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 5641 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa 5701 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg 5761 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct 5821 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca 5881 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta 5941 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc 6001 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc 6061 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag 6121 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg 6181 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg 6241 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta 6301 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc 6361 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt 6421 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta 6481 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat 6541 cagagagtga gaggccttga cattgctagc gttttaccgt cgacctctag ctagagcttg 6601 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 6661 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 6721 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 6781 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 6841 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 6901 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 6961 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 7021 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 7081 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 7141 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 7201 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 7261 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 7321 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 7381 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 7441 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 7501 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 7561 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 7621 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 7681 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 7741 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 7801 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 7861 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 7921 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 7981 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 8041 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 8101 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 8161 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 8221 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 8281 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 8341 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 8401 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 8461 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 8521 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 8581 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 8641 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 8701 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 8761 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata 8821 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 8881 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa 8941 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt 9001 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg 9061 ttaaatatgt actacaaact tagtagt //