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LOCUS pLVX-Hom-1 8478 bp DNA circular 5-OCT-2011 COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com COMMENT Catalog Nos. 635072, 635073 FEATURES Location/Qualifiers LTR 1..635 /label=5'\LTR misc_feature 685..822 /label=packaging\signal misc_feature 2028..2151 /label=cPPT/CTS misc_feature 4400..4991 /label=WPRE rep_origin complement(6301..6971) /label=pUC\ori CDS complement(7116..8112) /label=Amp(R) misc_binding 1303..1536 /label=RRE misc_binding 636..653 /label=PBS LTR 5195..5831 /label=3'LTR misc_feature 3179..3754 /label=IRES CDS 3787..4386 /label=Puro(R) misc_feature 2821..3153 /label=DmrB misc_feature 2185..2787 /label=pCMV BASE COUNT 2169 a 2111 c 2190 g 2008 t ORIGIN 1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt 2821 atggcttcta gaggagtgca ggtggagact atctccccag gagacgggcg caccttcccc 2881 aagcgcggcc agacctgcgt ggtgcactac accgggatgc ttgaagatgg aaagaaagtt 2941 gattcctccc gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc 3001 cgaggctggg aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata 3061 tctccagatt atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact 3121 ctcgtcttcg atgtggagct tctaaaactg gaaagcggcc gcggatccta cgtatgaccg 3181 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg 3241 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg 3301 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg 3361 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca 3421 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct 3481 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca 3541 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa 3601 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg 3661 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg 3721 ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaacccac aaggagacga 3781 ccttccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg 3841 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac 3901 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg 3961 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg 4021 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg 4081 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc 4141 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt 4201 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc 4261 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc 4321 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt 4381 gcctagacgc gtctggaaca atcaacctct ggattacaaa atttgtgaaa gattgactgg 4441 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 4501 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 4561 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 4621 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 4681 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 4741 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 4801 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 4861 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 4921 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc 4981 ctccccgcct ggaattaatt ctgcagtcga gacctagaaa aacatggagc aatcacaagt 5041 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag 5101 gtgggttttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt 5161 agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg 5221 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca 5281 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct 5341 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt 5401 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt 5461 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa 5521 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg 5581 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg 5641 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 5701 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 5761 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 5821 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat 5881 gaatatcaga gagtgagagg ccttgacatt gctagcgttt taccgtcgac ctctagctag 5941 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 6001 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 6061 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 6121 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 6181 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 6241 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 6301 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 6361 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 6421 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 6481 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 6541 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 6601 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 6661 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 6721 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 6781 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 6841 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt 6901 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 6961 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 7021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 7081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 7141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 7201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 7261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 7321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 7381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 7441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 7501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 7561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 7621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 7681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 7741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 7801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 7861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 7921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 7981 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 8041 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 8101 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa 8161 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 8221 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc 8281 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctagt 8341 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta 8401 tttgttaaat atgtactaca aacttagtag tttttaaaga aattgtattt gttaaatatg 8461 tactacaaac ttagtagt //