LOCUS       pCDF1-MCS2-EF1-Puro	6606 bp 	DNA	SYN
DEFINITION  pCDF1-MCS2-EF1-Puro
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="pCDF1-MCS2-EF1-Puro"
                     /mol_type="other DNA"
     misc_feature    287..307
                     /label="CMV_fwd_primer"
     misc_feature    1165..1180
                     /label="cPPT"
     misc_feature    1633..1653
                     /label="CMV_fwd_primer"
     promoter        1634..1703
                     /label="CMV_promoter"
     misc_feature    1677..1696
                     /label="pCEP_fwd_primer"
     misc_feature    1679..1703
                     /label="LNCX_primer"
     gene            2358..2957
                     /label="puro (variant)"
                     /gene="puro (variant)"
     CDS             2358..2957
                     /label="ORF frame 3"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     misc_feature    2966..3541
                     /label="WPRE"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     CDS             3054..3566
                     /label="ORF frame 3"
                     /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     SSSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGYR*"
     CDS             3067..3639
                     /label="ORF frame 1"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRLGTDDRVRRSLQEAIWHDFYNGRQHSRF*"
     misc_feature    3653..3678
                     /label="U3PPT"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRLGTDDRVRRSLQEAIWHDFYNGRQHSRF*"
     misc_feature    3655..3678
                     /label="U3PPT"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRLGTDDRVRRSLQEAIWHDFYNGRQHSRF*"
     terminator      3893..4012
                     /label="SV40_PA_terminator"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRLGTDDRVRRSLQEAIWHDFYNGRQHSRF*"
     misc_feature    3981..4000
                     /label="EBV_rev_primer"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRLGTDDRVRRSLQEAIWHDFYNGRQHSRF*"
     rep_origin      4030..4107
                     /label="SV40_origin"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRLGTDDRVRRSLQEAIWHDFYNGRQHSRF*"
     misc_feature    4092..4111
                     /label="SV40pro_F_primer"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRLGTDDRVRRSLQEAIWHDFYNGRQHSRF*"
     promoter        complement(4199..4217)
                     /label="M13_reverse_primer"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRLGTDDRVRRSLQEAIWHDFYNGRQHSRF*"
     misc_feature    complement(4216..4238)
                     /label="M13_pUC_rev_primer"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRLGTDDRVRRSLQEAIWHDFYNGRQHSRF*"
     promoter        complement(4252..4281)
                     /label="lac_promoter"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRLGTDDRVRRSLQEAIWHDFYNGRQHSRF*"
     gene            complement(5364..6224)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRLGTDDRVRRSLQEAIWHDFYNGRQHSRF*"
     CDS             complement(5364..6224)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(6266..6294)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(6453..6475)
                     /label="pGEX_3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
   61 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
  121 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
  181 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
  241 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
  301 aggcgtgtac ggtgggaggt ctatataagc agagcttgtg aaacttcgag gagtctcttt
  361 gttgaggact tttgagttct cccttgaggc tcccacagat acaataaata tttgagattg
  421 aaccctgtcg agtatctgtg taatcttttt tacctgtgag gtctcggaat ccgggccgag
  481 aacttcgcag ttggcgcccg aacagggact tgattgagag tgattgagga agtgaagcta
  541 gagcaataga aagctgttaa gcagaactcc tgctgaccta aatagggaag cagtagcaga
  601 cgctgctaac agtgagtatc tctagtgaag cagactcgag ctcataatca agtcattgtt
  661 taaaggccca gataaattac atctggtgac tcttcgcgga ccttcaagcc aggagattcg
  721 ccgagggaca gtcaacaagg taggagagat tctacagcaa catggggaat ggacaggggc
  781 gagattggaa aatggccatt aagagatgta gtaatgttgc tgtaggagta ggggggaaga
  841 gtaaaaaatt tggagaaggg aatttcagat gggccattag aatggctaat gtatctacag
  901 gacgagaacc tggtgatata ccagagactt tagatcaact aaggttggtt atttgcgatt
  961 tacaagaaag aagagaaaaa tttggatcta gcaaagaaat tgatatggca attcctgcat
 1021 tgaggagaaa tggtaggcaa tgtggcatgt ctgaaaaaga ggaggaatga tgaagtatct
 1081 cagacttatt ttataaggga gatactgtgc tgagttcttc cctttgagga aggtatgtca
 1141 tatcctagac atagtctcaa ttttaaaaga agaggtagga taggagggat ggccccttat
 1201 gaattattag cacaacaaga atccttaaga atacaagatt atttttctgc aataccacaa
 1261 aaattgcaag cacagtggat ttattataaa gatcaaaaag ataagaaatg gaaaggacca
 1321 atgagagtag aatactgggg acagggatca gtattattaa aggatgaaga gaagggatat
 1381 tttcttataa tcgatactag tattatgccc agtacatgac cttatgggac tttcctactt
 1441 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
 1501 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
 1561 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
 1621 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
 1681 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
 1741 gaagattcta gagcccgggc gcgccggatc cagatcttaa ttaatttaaa tgaattcgcg
 1801 gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
 1861 agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg
 1921 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
 1981 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
 2041 cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac
 2101 ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc
 2161 ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc
 2221 ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct
 2281 tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg
 2341 accggcgcct acgctagatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
 2401 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
 2461 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
 2521 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
 2581 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
 2641 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
 2701 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
 2761 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
 2821 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
 2881 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
 2941 gcaagcccgg tgcctgagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
 3001 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
 3061 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
 3121 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
 3181 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
 3241 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
 3301 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
 3361 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
 3421 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
 3481 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
 3541 ccgcctcccc gcctgggtac cgatgacaga gttagaagat cgcttcagga agctatttgg
 3601 cacgacttct acaacgggag acagcacagt agattctgaa gatgaacctc ctaaaaaaga
 3661 aaaaagggtg gactgggatg agtattggaa ccctgaaatc gatagcttcc agtgctttgt
 3721 gaaacttcga ggagtctctt tgttgaggac ttttgagttc tcccttgagg ctcccacaga
 3781 tacaataaat atttgagatt gaaccctgtc gagtatctgt gtaatctttt ttacctgtga
 3841 ggtctcggaa tccgggccga gaacttcgca gcgagctcat tgtaccgcga acttgtttat
 3901 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
 3961 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
 4021 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
 4081 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
 4141 tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca
 4201 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
 4261 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
 4321 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
 4381 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
 4441 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
 4501 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
 4561 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
 4621 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
 4681 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
 4741 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
 4801 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
 4861 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
 4921 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
 4981 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
 5041 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
 5101 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
 5161 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
 5221 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
 5281 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
 5341 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
 5401 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
 5461 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
 5521 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
 5581 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
 5641 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
 5701 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
 5761 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
 5821 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
 5881 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
 5941 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
 6001 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
 6061 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
 6121 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
 6181 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
 6241 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
 6301 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
 6361 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
 6421 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
 6481 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
 6541 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
 6601 gtgcac
//