LOCUS       pLVX-EF1a-mCherry-N1   9510 bp    DNA   circular            22-MAR-2010
COMMENT     Created by Clontech Laboratories Inc. 
COMMENT     Cat. No. 631986
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=Packaging\signal
     misc_feature    5478..6069
                     /label=WPRE
     CDS             complement(8196..9192)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             6272..6908
                     /label=3'\LTR
     promoter        4336..4844
                     /label=PGK\Promoter
     CDS             4865..5464
                     /label=Puro(R)
     CDS             3607..4317
                     /label=mCherry
     misc_feature    2028..2151
                     /label=cPPT/CTS\(DNA\flap)
     rep_origin      complement(7378..8051)
                     /label=pUC\origin
     promoter        2185..3519
                     /label=EF1a\Promoter
     misc_feature    3560..3599
                     /label=MCS
BASE COUNT     2254 a      2477 c      2621 g      2158 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatcgctagc gctaccggac 
     3541 tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca 
     3601 ccggtcatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc 
     3661 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc 
     3721 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc 
     3781 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg 
     3841 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg 
     3901 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg 
     3961 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc 
     4021 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag 
     4081 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac 
     4141 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac 
     4201 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag 
     4261 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtgagcg 
     4321 gccgcgactc tagataattc taccgggtag gggaggcgct tttcccaagg cagtctggag 
     4381 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca 
     4441 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc 
     4501 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca 
     4561 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg 
     4621 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt 
     4681 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg 
     4741 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca 
     4801 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct 
     4861 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc 
     4921 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc 
     4981 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct 
     5041 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc 
     5101 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag 
     5161 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa 
     5221 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct 
     5281 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt 
     5341 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac 
     5401 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc 
     5461 ctgaccgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta 
     5521 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 
     5581 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 
     5641 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 
     5701 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 
     5761 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 
     5821 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt 
     5881 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 
     5941 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 
     6001 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct 
     6061 ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag 
     6121 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt 
     6181 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga 
     6241 tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag 
     6301 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa 
     6361 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt 
     6421 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca 
     6481 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga 
     6541 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg 
     6601 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct 
     6661 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct 
     6721 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga 
     6781 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc 
     6841 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct 
     6901 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa 
     6961 tatcagagag tgagaggcct tgacattgct agcgttttac cgtcgacctc tagctagagc 
     7021 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 
     7081 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 
     7141 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 
     7201 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 
     7261 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 
     7321 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 
     7381 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 
     7441 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 
     7501 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 
     7561 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 
     7621 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 
     7681 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 
     7741 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 
     7801 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 
     7861 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 
     7921 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 
     7981 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 
     8041 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 
     8101 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 
     8161 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 
     8221 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 
     8281 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 
     8341 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 
     8401 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 
     8461 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 
     8521 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 
     8581 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 
     8641 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 
     8701 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 
     8761 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 
     8821 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 
     8881 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 
     8941 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 
     9001 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 
     9061 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 
     9121 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 
     9181 ccacctgacg tcgacggatc gggagatcaa cttgtttatt gcagcttata atggttacaa 
     9241 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 
     9301 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc 
     9361 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg 
     9421 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat 
     9481 ttgttaaata tgtactacaa acttagtagt 
//