LOCUS       pLVX-EF1a-AcGFP1-N1   9519 bp    DNA   circular            22-MAR-2010
COMMENT     Created by Clontech Laboratories Inc. 
COMMENT     Cat. No. 631983
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=Packaging\signal
     misc_feature    5487..6078
                     /label=WPRE
     rep_origin      complement(7387..8060)
                     /label=pUC\origin
     CDS             complement(8205..9201)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             6281..6917
                     /label=3'\LTR
     misc_feature    3528..3600
                     /label=MCS
     misc_feature    3607..4326
                     /label=AcGFP1
     misc_feature    4345..4853
                     /label=PGK\Promoter
     misc_feature    4874..5473
                     /label=Puro(R)
     promoter        2186..3519
                     /label=EF1a\Promoter
     misc_feature    2028..2151
                     /label=cPPT/CTS\(DNA\flap)
BASE COUNT     2264 a      2479 c      2598 g      2178 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg 
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc 
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct 
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt 
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt 
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatcgctagc gctaccggac 
     3541 tcagatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca 
     3601 ccggtcatgg tgagcaaggg cgccgagctg ttcaccggca tcgtgcccat cctgatcgag 
     3661 ctgaatggcg atgtgaatgg ccacaagttc agcgtgagcg gcgagggcga gggcgatgcc 
     3721 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc tgtgccctgg 
     3781 cccaccctgg tgaccaccct gagctacggc gtgcagtgct tctcacgcta ccccgatcac 
     3841 atgaagcagc acgacttctt caagagcgcc atgcctgagg gctacatcca ggagcgcacc 
     3901 atcttcttcg aggatgacgg caactacaag tcgcgcgccg aggtgaagtt cgagggcgat 
     3961 accctggtga atcgcatcga gctgaccggc accgatttca aggaggatgg caacatcctg 
     4021 ggcaataaga tggagtacaa ctacaacgcc cacaatgtgt acatcatgac cgacaaggcc 
     4081 aagaatggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggatgg cagcgtgcag 
     4141 ctggccgacc actaccagca gaataccccc atcggcgatg gccctgtgct gctgcccgat 
     4201 aaccactacc tgtccaccca gagcgccctg tccaaggacc ccaacgagaa gcgcgatcac 
     4261 atgatctact tcggcttcgt gaccgccgcc gccatcaccc acggcatgga tgagctgtac 
     4321 aagtgagcgg ccgcgactct agataattct accgggtagg ggaggcgctt ttcccaaggc 
     4381 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct 
     4441 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc 
     4501 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg 
     4561 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca 
     4621 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct 
     4681 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg 
     4741 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc 
     4801 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca 
     4861 gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt 
     4921 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac 
     4981 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg 
     5041 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg 
     5101 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc 
     5161 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca 
     5221 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg 
     5281 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt 
     5341 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt 
     5401 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa 
     5461 gcccggtgcc tgaccgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat 
     5521 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 
     5581 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 
     5641 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 
     5701 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 
     5761 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 
     5821 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 
     5881 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt 
     5941 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 
     6001 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 
     6061 gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat 
     6121 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga 
     6181 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc 
     6241 agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc 
     6301 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga 
     6361 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta 
     6421 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag 
     6481 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg 
     6541 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt 
     6601 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag 
     6661 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc 
     6721 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct 
     6781 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt 
     6841 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt 
     6901 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa 
     6961 agaaatgaat atcagagagt gagaggcctt gacattgcta gcgttttacc gtcgacctct 
     7021 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 
     7081 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 
     7141 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 
     7201 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 
     7261 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
     7321 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 
     7381 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     7441 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     7501 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     7561 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     7621 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     7681 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     7741 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     7801 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     7861 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 
     7921 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     7981 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
     8041 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
     8101 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 
     8161 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 
     8221 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 
     8281 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 
     8341 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 
     8401 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 
     8461 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 
     8521 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 
     8581 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 
     8641 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 
     8701 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 
     8761 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 
     8821 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 
     8881 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 
     8941 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 
     9001 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 
     9061 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
     9121 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
     9181 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa 
     9241 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 
     9301 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat 
     9361 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa 
     9421 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag 
     9481 aaattgtatt tgttaaatat gtactacaaa cttagtagt 
//