LOCUS       pSicoR	7567 bp 	DNA     circular	SYN
DEFINITION  pSicoR
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..7567
                     /organism="pSicoR"
                     /mol_type="other DNA"
     promoter        248..824
                     /label="CMV_immearly_promoter"
     misc_feature    327..614
                     /label="CAG_enhancer"
     misc_feature    781..801
                     /label="CMV_fwd_primer"
     misc_feature    844..1024
                     /label="HIV-1_5_LTR"
     misc_feature    844..1024
                     /label="truncHIV-1_3_LTR"
     misc_feature    1135..1179
                     /label="HIV-1_psi_pack"
     CDS             1573..2460
                     /label="ORF frame 1"
     misc_feature    1695..1928
                     /label="RRE"
     misc_feature    2459..2474
                     /label="cPPT"
     misc_feature    2826..2859
                     /label="loxP"
     promoter        3025..3577
                     /label="CMV_immearly_promoter"
     misc_feature    3080..3367
                     /label="CAG_enhancer"
     misc_feature    3534..3554
                     /label="CMV_fwd_primer"
     promoter        3535..3604
                     /label="CMV_promoter"
     CDS             complement(3613..4383)
                     /label="ORF frame 2"
     CDS             3628..4347
                     /label="ORF frame 1"
     gene            3631..4344
                     /label="EGFP"
                     /gene="EGFP"
     misc_feature    complement(3673..3694)
                     /label="EGFP_N_primer"
     misc_feature    3811..3840
                     /label="Y66 (EGFP)"
     misc_feature    4281..4302
                     /label="EGFP_C_primer"
     misc_feature    4367..4400
                     /label="loxP"
     misc_feature    4458..5045
                     /label="WPRE"
     CDS             4546..5100
                     /label="ORF frame 1"
     CDS             4559..5599
                     /label="ORF frame 2"
     misc_feature    complement(5048..5064)
                     /label="pBluescriptKS_primer"
     misc_feature    5236..5257
                     /label="U3PPT"
     misc_feature    5236..5251
                     /label="cPPT"
     misc_feature    5573..5753
                     /label="HIV-1_5_LTR"
     misc_feature    5573..5753
                     /label="truncHIV-1_3_LTR"
     gene            complement(6574..7434)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(6574..7434)
                     /label="ORF frame 2"
     promoter        complement(7476..7504)
                     /label="AmpR_promoter"
ORIGIN
    1 gcttaagcgg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
   61 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
  121 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
  181 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
  241 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
  301 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
  361 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
  421 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
  481 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
  541 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
  601 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
  661 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
  721 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
  781 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
  841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
  901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
  961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
 1021 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
 1081 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
 1141 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
 1201 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
 1261 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
 1321 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
 1381 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
 1441 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
 1501 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgcgctgat cttcagacct
 1561 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
 1621 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
 1681 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
 1741 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
 1801 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
 1861 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
 1921 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
 1981 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
 2041 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
 2101 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
 2161 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
 2221 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
 2281 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
 2341 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
 2401 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
 2461 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
 2521 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
 2581 ttacagggac agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc
 2641 cgccatctct aggcccgcgc cggccccctc gcacagactt gtgggagaag ctcggctact
 2701 cccctgcccc ggttaatttg catataatat ttcctagtaa ctatagaggc ttaatgtgcg
 2761 ataaaagaca gataatctgt tctttttaat actagctaca ttttacatga taggcttgga
 2821 tttctataac ttcgtatagc atacattata cgaagttata catgtcacaa aaggaaactc
 2881 accctaactg taaagtaatt gtgtgttttg agactataaa tatcccttgg agaaaagcct
 2941 tgttaacgcg cggtgaccct cgagtactag gatccattag gcggccgcgt ggataaccgt
 3001 attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc
 3061 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
 3121 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
 3181 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
 3241 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
 3301 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
 3361 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
 3421 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
 3481 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
 3541 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
 3601 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
 3661 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
 3721 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
 3781 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
 3841 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
 3901 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
 3961 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
 4021 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
 4081 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
 4141 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
 4201 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
 4261 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
 4321 ctcggcatgg acgagctgta caagtaggaa ttcgtcgagg gacctaataa cttcgtatag
 4381 catacattat acgaagttat acatgtttaa gggttccggt tccactaggt acaattcgat
 4441 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
 4501 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
 4561 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
 4621 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
 4681 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
 4741 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
 4801 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
 4861 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
 4921 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
 4981 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
 5041 ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa catggagcaa tcacaagtag
 5101 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
 5161 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
 5221 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
 5281 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
 5341 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
 5401 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
 5461 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
 5521 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
 5581 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
 5641 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
 5701 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatgtg
 5761 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
 5821 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
 5881 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
 5941 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
 6001 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
 6061 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
 6121 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
 6181 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
 6241 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
 6301 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
 6361 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
 6421 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
 6481 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
 6541 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
 6601 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
 6661 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
 6721 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
 6781 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
 6841 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
 6901 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
 6961 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
 7021 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
 7081 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
 7141 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
 7201 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
 7261 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
 7321 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
 7381 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
 7441 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
 7501 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
 7561 acctgac
//